CBL196 | Anti-Cytokeratin 10 Antibody, clone LH-2

CBL196
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL196
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 10 Antibody, clone LH-2
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationDetect Cytokeratin 10 using this Anti-Cytokeratin 10 Antibody, clone LH-2 validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesIdentification of cytokeratin expressing cells by indirect immmunoperoxidase staining of frozen sections

      Western blotting

      IHC - formalin fixed sections. Pretreatment with type XXIV protease (0.5mg/ml) produces best results.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      CloneLH-2
      HostMouse
      SpecificityThe antibody reacts specifically with human cytokeratin 10 found in suprabasal cells of stratified squamous epithelia.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
      Gene Symbol
      • KRT10
      • K10
      • CK-10
      • Keratin-10
      • Cytokeratin-10
      • KPP
      • CK10
      UniProt Number
      UniProt SummarySIZE: 593 amino acids; 59511 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-10 is generally associated with keratin-1.
      TISSUE SPECIFICITY: Seen in all suprabasal cell layers including stratum corneum.
      DISEASE: SwissProt: P13645 # Defects in KRT10 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. & Defects in KRT10 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT10 are a cause of epidermal nevus epidermolytic hyperkeratotic type [MIM:600648]. Epidermal nevi affect about 1 in 1,000 people. They appear at or shortly after birth as localized lines of epidermal thickening. The extent of skin involvement varies widely.
      SIMILARITY: SwissProt: P13645 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CYTOKERATIN 10 2485715
      MOUSE ANTI-HUMAN CYTOKERATIN 10 MONOCLONAL ANTIBODY - 2318791 2318791
      MOUSE ANTI-HUMAN CYTOKERATIN 10 MONOCLONAL ANTIBODY - 2434446 2434446
      MOUSE ANTI-HUMAN CYTOKERATIN 10 MONOCLONAL ANTIBODY - 2074770 2074770
      MOUSE ANTI-HUMAN CYTOKERATIN 10 MONOCLONAL ANTIBODY - 2201457 2201457

      References

      Reference overviewPub Med ID
      Expression of simple epithelial keratins 8 and 18 in epidermal neoplasia.
      Markey, A C, et al.
      J. Invest. Dermatol., 97: 763-70 (1991)  1991

      Show Abstract
      1717607 1717607
      An investigation of cytokeratin expression in skin epithelial cysts and some uncommon types of cystic tumours using chain-specific antibodies.
      Broekaert, D, et al.
      Arch. Dermatol. Res., 282: 383-91 (1990)  1990

      Show Abstract
      1701984 1701984

      Data Sheet

      Title
      Anti-Cytokeratin 10, clone LH-2 - Data Sheet

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MAB3230 Anti-Cytokeratin 10 Antibody, clone RKSE60 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies