04-587 | Anti-Cytokeratin 5 Antibody

04-587
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue Number04-587
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cytokeratin 5 Antibody
      Background InformationCytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
      Applications
      ApplicationAnti-Cytokeratin 5 Antibody detects level of Cytokeratin 5 & has been published & validated for use in WB, IC, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWB: 1:1000
      IHC: 1:50 - 100 (Paraffin-embedded human transitional carcinoma of bladder)
      ICC: 1:50 - 100
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human CK-5.
      HostRabbit
      SpecificityRecognizes Human Cytokeratin 5.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman (positive control: A431 cell lysate)
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
      Gene Symbol
      • KRT5
      • DDD
      • Keratin-5
      • KRT5A
      • CK-5
      • CK5
      • K5
      • EBS2
      • Cytokeratin-5
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummarySIZE: 590 amino acids; 62378 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
      DISEASE: SwissProt: P13647 # Defects in KRT5 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 131960]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%) and it is characterized by blistering within the basal cell layer. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal-epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema [MIM:609352]. EBS with migratory circinate erythema is milder than the DM-EBS phenotype but involves an unusual migratory circinate erythema with multiple vesicles on the area affected by the erythema. The lesions, which appeared from birth primarily on the hands, feet, and legs but spared the nails, ocular epithelia, and mucosae, healed with brown pigmentation but no scarring. Electron microscopy findings were distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is characterized by additional reticular hyperpigmentation. & Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
      SIMILARITY: SwissProt: P13647 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 2386944 2386944
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 2386949 2386949
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 1962571 1962571
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 2022619 2022619
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 2168260 2168260
      Anti-Cytokeratin 5 (Rabbit polyclonal) - 2349459 2349459
      Anti-Cytokeratin 5 - DAM1423798 DAM1423798
      Anti-Cytokeratin 5 - DAM1581054 DAM1581054
      Anti-Cytokeratin 5 - NG1660758 NG1660758

      References

      Reference overviewPub Med ID
      Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin.
      Lönnqvist, S; Rakar, J; Briheim, K; Kratz, G
      PloS one  10  e0128093  2015

      Show Abstract
      26061630 26061630
      Expression of cytokeratin 5 and calretinin in clear-cell renal cell carcinoma
      Cid Mouteira, P, et al
      Actas urologicas españolas , 30:367-71 (2006)  2006

      16838608 16838608
      Isolation, sequence, and expression of a human keratin K5 gene: transcriptional regulation of keratins and insights into pairwise control.
      Lersch, R, et al.
      Mol. Cell. Biol., 9: 3685-97 (1989)  1989

      Show Abstract
      2476664 2476664

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies