04-588 | Anti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432

04-588
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, WB, ICC, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-588
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432
      Background InformationKeratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Cytokeratin 8 using this Anti-Cytokeratin 8 Antibody, Rabbit, clone E432 validated for use in FC, WB, IC, IH(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWB: 1:500
      IHC: 1:250-1:500 (Paraffin-embedded stomach carcinoma)
      ICC: 1:50
      Flow Cytometry: 1:20
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8.
      CloneE432
      HostRabbit
      SpecificityRecognizes Human, Mouse Cytokeratin 8.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse (positive control: A431 cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT8
      • Cytokeratin-8
      • Keratin-8
      • K2C8
      • CYK8
      • CK-8
      • KO
      • K8
      • CK8
      • CARD2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
      SIZE: 483 amino acids; 53704 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
      PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      DISEASE:SwissProt: P05787 # Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].
      SIMILARITY: SwissProt: P05787 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cytokeratin 8, clone E432 - 2424478 2424478
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2151807 2151807
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2387747 2387747
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2397045 2397045
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 1959200 1959200
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2090431 2090431
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2318071 2318071
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - 2343134 2343134
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - DAM1447146 DAM1447146
      Anti-Cytokeratin 8, clone E432 (Rabbit Monoclonal) - NG1938135 NG1938135

      References

      Reference overviewPub Med ID
      Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
      Muchkaeva, IA; Dashinimaev, EB; Artyuhov, AS; Myagkova, EP; Vorotelyak, EA; Yegorov, YY; Vishnyakova, KS; Kravchenko, IE; Chumakov, PM; Terskikh, VV; Vasiliev, AV
      Acta naturae  6  45-53  2014

      Show Abstract
      24772326 24772326
      Keratins and the skin.
      Fuchs, E
      Annu. Rev. Cell Dev. Biol., 11: 123-53 (1995)  1995

      Show Abstract
      8689554 8689554
      Identification and mutational analysis of the glycosylation sites of human keratin 18
      Ku, N O and Omary, M B
      J Biol Chem, 270:11820-7 (1995)  1995

      7538124 7538124
      Cytokeratin expression, fibrillar organization, and subtle function in liver cells
      Marceau, N and Loranger, A
      Biochem Cell Biol, 73:619-25 ()  1995

      8714681 8714681
      Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells
      Kulesh, D A and Oshima, R G
      Mol Cell Biol, 8:1540-50 (1988)  1988

      2454392 2454392

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      Life Science Research > Antibodies and Assays > Primary Antibodies