07-2050 | Anti-DNMT3A2 Antibody

07-2050
400 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, ELISA, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2050
      DescriptionAnti-DNMT3A2 Antibody
      Alternate Names
      • DNA (cytosine-5-)-methyltransferase 3 alpha
      • DNA MTase HsaIIIA
      • DNA cytosine methyltransferase 3 alpha
      • DNA cytosine methyltransferase 3A2
      • DNA methyltransferase HsaIIIA
      Background InformationCpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation.
      References
      Product Information
      FormatPurified
      Control
      • L6, NIH/3T3, HeLa, or A431 cells.
      PresentationPurified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
      Applications
      ApplicationUse Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha.
      Key Applications
      • Immunocytochemistry
      • ELISA
      • Western Blotting
      Application Notes ELISA:
      This antibody was shown by an outside laboratory to be suitable for ELISA.

      Immunocytochemistry:
      This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
      Gene Symbol
      • DNMT3A2
      • Dnmt3a
      • EC 2.1.1.37
      • M.HsaIIIA
      • OTTHUMP00000120005
      • OTTHUMP00000120006
      Modifications
      • Methylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. Ref.10

      Catalytic activity S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

      SUBUNIT STRUCTURE: Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger By similarity. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Ref.3

      TISSUE SPECIFICITY: Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. Ref.7

      Sequence similarities Belongs to the C5-methyltransferase family.

      Contains 1 ADD-type zinc finger.

      Contains 1 PWWP domain.

      CAUTION:It is uncertain whether Met-1 or Met-4 is the initiator

      Molecular Weight~80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size400 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-DNMT3A2 - 2375580 2375580
      Anti-DNMT3A2 - 1962090 1962090
      Anti-DNMT3A2 - 1963090 1963090
      Anti-DNMT3A2 - 2058962 2058962
      Anti-DNMT3A2 - 2090949 2090949
      Anti-DNMT3A2 - 2363638 2363638
      Anti-DNMT3A2 - 2510376 2510376
      Anti-DNMT3A2 - NG1593369 NG1593369
      Anti-DNMT3A2 - NG1842818 NG1842818
      Anti-DNMT3A2 -2763991 2763991

      References

      Reference overviewPub Med ID
      Reconstitution and mechanism of the stimulation of de novo methylation by human DNMT3L.
      Kareta, Michael S, et al.
      J. Biol. Chem., 281: 25893-902 (2006)  2006

      16829525 16829525
      Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation.
      Nimura, Keisuke, et al.
      Genes Cells, 11: 1225-37 (2006)  2006

      16999741 16999741
      A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
      Chen, Taiping, et al.
      J. Biol. Chem., 277: 38746-54 (2002)  2002

      12138111 12138111