07-471 | Anti-Daxx Antibody

07-471
200 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, M, RIP, WB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-471
      Replaces04-445
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Daxx Antibody
      References
      Product Information
      FormatSerum
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-Daxx Antibody is an antibody against Daxx for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 472-740 of human Daxx.
      HostRabbit
      SpecificityDaxx
      IsotypeIgG
      Species Reactivity
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DAXX
      • MGC126246
      • Daxx
      • BING2
      • hDaxx
      • MGC126245
      • EAP1
      • DAP6
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UER7 # Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
      SIZE: 740 amino acids; 81373 Da
      SUBUNIT: Homomultimer. Binds to the TNFRSF6 death domain via its C-terminus and to PAX5. Binds to SLC2A4/GLUT4, MAP3K5, TGFBR2, phosphorylated dimeric HSPB1/HSP27, CENPC1, ETS1, sumoylated PML, UBE2I and MCRS1. Is part of a complex containing PAX5 and CREBBP. Interacts with HIPK2 and HIPK3 via its N-terminus. Interacts with HIPK1, which induces translocation from PML/POD/ND10 nuclear bodies to chromatin and enhances association with HDAC1 (By similarity). The non-phosphorylated form binds to PAX3, PAX7, DEK, HDAC1, HDAC2, HDAC3, acetylated histone H4 and histones H2A, H2B, H3 and H4. Interacts with SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress.TISSUE SPECIFICITY: Ubiquitous.
      PTM: Sumoylated. & Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by HIPK1 upon glucose deprivation. & Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation.
      SIMILARITY: SwissProt: Q9UER7 ## Belongs to the DAXX family.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Daxx (rabbit antiserum) 2931110
      Anti-Daxx (rabbit antiserum) -2828854 2828854
      Anti-Daxx - 24496 24496
      Anti-Daxx - 3019771 3019771
      Anti-Daxx_2841952 2841952

      References

      Reference overviewApplicationSpeciesPub Med ID
      Influence of ND10 components on epigenetic determinants of early KSHV latency establishment.
      Günther, T; Schreiner, S; Dobner, T; Tessmer, U; Grundhoff, A
      PLoS pathogens  10  e1004274  2014

      Show Abstract
      25033267 25033267
      Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX chromatin-associated complexes.
      Schreiner, S; Bürck, C; Glass, M; Groitl, P; Wimmer, P; Kinkley, S; Mund, A; Everett, RD; Dobner, T
      Nucleic acids research  41  3532-50  2013

      Show Abstract
      23396441 23396441
      The replication defect of ICP0-null mutant herpes simplex virus 1 can be largely complemented by the combined activities of human cytomegalovirus proteins IE1 and pp71.
      Everett, RD; Bell, AJ; Lu, Y; Orr, A
      Journal of virology  87  978-90  2013

      Show Abstract
      Immunofluorescence23135716 23135716
      The viral ubiquitin ligase ICP0 is neither sufficient nor necessary for degradation of the cellular DNA sensor IFI16 during herpes simplex virus 1 infection.
      Cuchet-Lourenço, D; Anderson, G; Sloan, E; Orr, A; Everett, RD
      Journal of virology  87  13422-32  2013

      Show Abstract
      Immunofluorescence24089555 24089555
      Components of promyelocytic leukemia nuclear bodies (ND10) act cooperatively to repress herpesvirus infection.
      Glass, M; Everett, RD
      Journal of virology  87  2174-85  2013

      Show Abstract
      Immunofluorescence23221561 23221561
      Herpes simplex virus 1 ubiquitin ligase ICP0 interacts with PML isoform I and induces its SUMO-independent degradation.
      Cuchet-Lourenço, D; Vanni, E; Glass, M; Orr, A; Everett, RD
      Journal of virology  86  11209-22  2012

      Show Abstract
      22875967 22875967
      Single-cell analysis of Daxx and ATRX-dependent transcriptional repression.
      Newhart, A; Rafalska-Metcalf, IU; Yang, T; Negorev, DG; Janicki, SM
      Journal of cell science  125  5489-501  2012

      Show Abstract
      Western Blotting22976303 22976303
      Adenovirus type 5 early region 1B 55K oncoprotein-dependent degradation of cellular factor Daxx is required for efficient transformation of primary rodent cells.
      Schreiner, S; Wimmer, P; Groitl, P; Chen, SY; Blanchette, P; Branton, PE; Dobner, T
      Journal of virology  85  8752-65  2011

      Show Abstract
      21697482 21697482
      Human cytomegalovirus immediate early gene expression in the osteosarcoma line U2OS is repressed by the cell protein ATRX.
      Steven McFarlane,Chris M Preston
      Virus research  157  2011

      Show Abstract
      21310198 21310198
      SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes.
      Cuchet-Lourenço, D; Boutell, C; Lukashchuk, V; Grant, K; Sykes, A; Murray, J; Orr, A; Everett, RD
      PLoS pathogens  7  e1002123  2011

      Show Abstract
      Human21779164 21779164

      Related Products & Applications

      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies