04-445 | Anti-Daxx Antibody, rabbit monoclonal

04-445
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-445
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Daxx Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Daxx Antibody, Rabbit is validated for use in FC, IF, WB, IH(P) for the detection of Daxx.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide composed of residues of human Daxx.
      HostRabbit
      SpecificityRecognizes a region composed of residues of human Daxx.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DAXX
      • MGC126246
      • Daxx
      • BING2
      • hDaxx
      • MGC126245
      • EAP1
      • DAP6
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UER7 # Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
      SIZE: 740 amino acids; 81373 Da
      SUBUNIT: Homomultimer. Binds to the TNFRSF6 death domain via its C-terminus and to PAX5. Binds to SLC2A4/GLUT4, MAP3K5, TGFBR2, phosphorylated dimeric HSPB1/HSP27, CENPC1, ETS1, sumoylated PML, UBE2I and MCRS1. Is part of a complex containing PAX5 and CREBBP. Interacts with HIPK2 and HIPK3 via its N-terminus. Interacts with HIPK1, which induces translocation from PML/POD/ND10 nuclear bodies to chromatin and enhances association with HDAC1 (By similarity). The non-phosphorylated form binds to PAX3, PAX7, DEK, HDAC1, HDAC2, HDAC3, acetylated histone H4 and histones H2A, H2B, H3 and H4. Interacts with SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress.TISSUE SPECIFICITY: Ubiquitous.
      PTM: Sumoylated. & Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by HIPK1 upon glucose deprivation. & Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation.
      SIMILARITY: SwissProt: Q9UER7 ## Belongs to the DAXX family.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Daxx 2473580
      Anti-Daxx (Rabbit monoclonal IgG) - 2446294 2446294
      Anti-Daxx (Rabbit monoclonal IgG) - 2460921 2460921
      Anti-Daxx (Rabbit monoclonal IgG) - 1990680 1990680
      Anti-Daxx (Rabbit monoclonal IgG) - 2000367 2000367
      Anti-Daxx (Rabbit monoclonal IgG) - 2032185 2032185
      Anti-Daxx (Rabbit monoclonal IgG) - 2208753 2208753
      Anti-Daxx (Rabbit monoclonal IgG) -2504286 2504286
      Anti-Daxx - 1945549 1945549
      Anti-Daxx - 2059853 2059853

      References

      Reference overviewPub Med ID
      Promyelocytic leukemia protein (PML) and Daxx participate in a novel nuclear pathway for apoptosis.
      Zhong, S, et al.
      J. Exp. Med., 191: 631-40 (2000)  2000

      Show Abstract
      10684855 10684855
      Daxx, a novel Fas-binding protein that activates JNK and apoptosis
      Yang, X, et al
      Cell, 89:1067-76 (1997)  1997

      9215629 9215629

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies