CBL173 | Anti-Desmoplakin 1& 2 Antibody, clone DP2.15

CBL173
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, RIF, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL173
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Desmoplakin 1& 2 Antibody, clone DP2.15
      References
      Product Information
      FormatPurified
      PresentationLyophilized. Reconstitute in 1 mL distilled water (final solution contains 0.09% sodium
      azide, 0.5% BSA in PBS buffer, pH 7.4
      Applications
      ApplicationAnti-Desmoplakin 1& 2 Antibody, clone DP2.15 detects level of Desmoplakin 1& 2 & has been published & validated for use in IF, WB & IC.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot
      Immunohistochemistry: 1:10; Frozen tissue
      Immunofluorescence

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenBovine desmoplakin 1 & 2
      CloneDP2.15
      HostMouse
      SpecificityThis antibody shows distinct punctate membrane staining of desmoplakins 1 (MW 250 kDa) and 2 (MW 215 kDa) in stratified epithelia and simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDesmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
      Gene Symbol
      • DSP
      • DPI
      • Desmoplakin
      • PPKS2
      • KPPS2
      • DP
      • DPII
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15924 # Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
      SIZE: 2871 amino acids; 331774 Da
      SUBUNIT: Homodimer.
      SUBCELLULAR LOCATION: Note=Innermost portion of the desmosomal plaque.
      TISSUE SPECIFICITY: Isoform DPI is apparently an obligate constituent of all desmosomes; Isoform DPII resides predominantly in tissues and cells of stratified origin.
      DOMAIN: SwissProt: P15924 The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1. The C-terminal region interacts with intermediate filaments.
      PTM: Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide.
      DISEASE: "SwissProt: P15924 # Defects in DSP are the cause of keratosis palmoplantaris striata II (PPKS2) [MIM:125647]; also known as striate palmoplantar keratoderma II (SPPK2). PPKS2 is characterized by skin thickening in the palms (linear pattern) and the soles (island-like pattern) and flexor aspect of the fingers; and rarely by abnormalities of the nails, the teeth and the hair. & Defects in DSP are the cause of dilated cardiomyopathy with woolly hair and keratoderma (DCWHK) [MIM:605676]; also known as Carvajal syndrome or palmoplantar keratoderma with left ventricular cardiomyopathy and woolly hair. DCWHK is an autosomal recessive cardiocutaneous syndrome characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy. & Defects in DSP are the cause of familial arrhythmogenic right ventricular dysplasia 8 (ARVD8) [MIM:607450]; also known as arrhythmogenic right ventricular cardiomyopathy 8 (ARVC8). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in DSP are the cause of skin fragility-woolly hair syndrome (SFWHS) [MIM:607655]. SFWHS is an autosomal recessive genodermatosis characterized by focal and diffuse palmoplantar keratoderma, hyperkeratotic plaques on the trunk and limbs, and woolly hair with varying degrees of alopecia. & Defects in DSP are the cause of lethal acantholytic epidermolysis bullosa [MIM:609638]. Lethal acantholytic epidermolysis bullosa is characterized by severe fragility of skin and mucous membranes. The phenotype is lethal in the neonatal period because of immense transcutaneous fluid loss. Typical features include universal alopecia, neonatal teeth, and nail loss. Histopathology of the skin shows suprabasal clefting and acantholysis throughout the spinous layer, mimicking pemphigus."
      SIMILARITY: Belongs to the plakin or cytolinker family. & Contains 17 plectin repeats. & Contains 2 spectrin repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsReconstituted antibody can be stored at 2°-8°C for up to 12 months from date of receipt. Antibody can also be stored at -20°C in undiluted aliquots.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 2 MONOCLONAL ANTIBODY - 2136580 2136580
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 2843082
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 MONOCLONAL ANTIBODY - 2366505 2366505
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 MONOCLONAL ANTIBODY - 2428482 2428482
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 - 2990640 2990640
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 -2584042 2584042
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 -2636719 2636719
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 -2772786 2772786
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 -2812014 2812014
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 MONOCLONAL ANTIBODY 2941116

      References

      Reference overviewPub Med ID
      Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins.
      Franke, W W, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 543-7 (1983)  1983

      Show Abstract
      6340102 6340102

      Data Sheet

      Title
      MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 MONOCLONAL ANTIBODY

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MABT513 Anti-Desmoplakin , clone EPR4383(2), Rabbit Monoclonal Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies