Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|B, Ch, H, M, R||IF, WB, ICC||M||Purified||Monoclonal Antibody|
|Description||Anti-Desmoplakin 1& 2 Antibody, clone DP2.15|
|Presentation||Lyophilized. Reconstitute in 1 mL distilled water (final solution contains 0.09% sodium
azide, 0.5% BSA in PBS buffer, pH 7.4
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Reconstituted antibody can be stored at 2°-8°C for up to 12 months from date of receipt. Antibody can also be stored at -20°C in undiluted aliquots.|
|Material Size||50 µg|
|Reference overview||Pub Med ID|
|Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins.|
Franke, W W, et al.
Proc. Natl. Acad. Sci. U.S.A., 80: 543-7 (1983) 1983
Epithelial cells contain desmosomes, special intercellular junctions providing sites of membrane attachment for intermediate-sized filaments of the cytokeratin type (tonofilaments). Such sites of anchorage of tonofilaments appear as dense plaques on the cytoplasmic side of the desmosomal membrane. We have isolated desmosome-enriched fractions from bovine snout epidermis and tongue mucosa and have characterized the major protein associated with the desmosomal plaque. This protein occurs in equimolar amounts of two polypeptides of Mr 250,000 (desmoplakin I) and Mr 215,000 (desmoplakin II) which are chemically and immunologically related. Antibodies raised against desmoplakins allow the identification and localization of this protein in epithelial cells grown in tissues or in vitro and show crossreaction in species as diverse as man, mouse, and chicken. Using immunolocalization at the light and electron microscope levels, we show that these antibodies bind specifically to desmosomal plaques. Antibodies to desmoplakins have been used successfully for detection of desmosomal proteins in a broad variety of epithelium-derived human tumors, including primary carcinomas and their metastases, irrespective of the morphology of the specific tumor. Nonepithelial tumors examined have been negative. We propose to use antibodies to desmoplakins and to cytokeratins in pathological diagnosis as two independent markers for the positive immunocytochemical identification and classification of epithelium derived tumors.
|MOUSE ANTI-HUMAN DESMOPLAKIN 1 & 2 MONOCLONAL ANTIBODY|