06-1062 | Anti-Deubiquitinating enzyme A (DUBA) Antibody

06-1062
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ca, B, Chp, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1062
      DescriptionAnti-Deubiquitinating enzyme A (DUBA) Antibody
      Alternate Names
      • Deubiquinating enzyme A
      • OTU domain containing 5
      Background InformationDeubiquitinating enzyme A (DUBA), an ovarian tumor domain-containing deubiquitinating enzyme, is a negative regulator of innate immune system and a regulator of type I interferon production. DUBA's deubiquitinating activity is directed towards 'Lys-63'-linked polyubiquitin chains and can be inhibited by N-ethyl-maleimide (NEM). DUBA binds to tumor necrosis factor receptor–associated factor 3 (TRAF3), an adaptor protein essential for type I interferon response. TRAF3 is an E3 ubiquitin ligase that preferentially assembles lysine-63–linked polyubiquitin chains. DUBA selectively cleaves the lysine-63–linked polyubiquitin chains on TRAF3, resulting in its dissociation from the downstream signaling complex containing TANK-binding kinase 1. (Kayagaki, N., et al., 2007)
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Deubiquitinating enzyme A (DUBA) Antibody detects level of Deubiquitinating enzyme A (DUBA) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: 1 µg/mL from a previous lot detected Deubiquitinating enzyme A (DUBA) on 10 µg of NIH/3T3 cell lysate.

      Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected Deubiquitinating enzyme A (DUBA) on 10 µg of Jurkat cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Deubiquitinating enzyme A (DUBA).
      EpitopeRecognizes all Isoforms of Deubiquitinating enzyme A (DUBA).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Deubiquitinating enzyme A (DUBA).
      Species Reactivity
      • Human
      • Mouse
      • Canine
      • Bovine
      • Chimpanzee
      • Rat
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Predicted to react with canine, bovine, chimpanzee, and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
      Gene Symbol
      • DUBA
      • OTUD5
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.

      CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

      ENZYME REGULATION: Inhibited by N-ethyl-maleimide (NEM).

      SUBUNIUT STRUCTURE: Interacts with TRAF3.

      TISSUE SPECIFICITY: Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.

      INDUCTION: By bacterial lipopolysaccharides (LPS) in bone marrow-derived macrophages.

      SEQUENCE SIMILARTIES: Belongs to the peptidase C85 family.

      Contains 1 OTU domain.
      Molecular Weight~ 62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Deubiquitinating enzyme A (DUBA) on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications