05-1338 | Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303

05-1338
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, VrtWB, IP, DB, ICC, ELISA, Mplex, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1338
      DescriptionAnti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303
      Alternate Names
      • H3K4me2
      • Histone H3 (di methyl K4)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Dimethyl-lysine 4 histone H3 (H3K4me2) is a transcription-activating chromatin mark, and dimethyl histone H3 Lys4 (H3K4me2) is depleted from regions with DNA methylation. Multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent and highly enriched in lineage-specific hematopoietic genes, suggesting a role for H3K4 methylation in differentiation.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Acid extract lysate
      PresentationPurified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303 is a mouse monoclonal antibody for detection of Dimethyl Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4). Demonstrated performance in ChIP, DB, ELISA, ICC, IP, WB, Mplex.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Dot Blot
      • Immunocytochemistry
      • ELISA
      • Multiplexing
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, or Anti-dimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G (Cat. # 17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys4) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH Coding.
      Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

      Dot Blot Analysis:
      Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 05-1338, Anti-dimethyl H3 (Lys4), clone CMA303 at 2.0 µg/mL (1:500 dilution). Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

      ChIP-seq Analysis:
      Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 µg of Anti-dimethyl-Histone H3 (Lys4) antibody (cat# 05-1338), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of sixteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 04-790 and 05-1338 datasets showed 92 and 90% overlap with peaks identified in the ENCODE H3K4me2 BROAD Histone track for HeLa S3.

      Immunocytochemistry:
      This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.

      Immunoprecipitation:
      This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.

      ELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA

      Multiplexing:
      This antibody specifically recognizes histone H3 dimethylated on Lys4 by Luminex® assay.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH.
      EpitopeDimethylated Lys4
      CloneCMA303
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody specifically recognizes Histone H3 dimethylated at Lys4. The antibody binding specificity allows for phosphorylation of Thr3 and/or modifications of Lys91.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3.4
      • H3/g
      • H3/t
      • H3FT
      • H3T
      • H3t
      • MGC126886
      • MGC126888
      • OTTHUMP00000037945
      Modifications
      • Methylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed in testicular cells.

      Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM:Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.

      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.

      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

      Ubiquitinated By similarity.

      SIMILARITY: Belongs to the histone H3 family.

      Molecular Weightapprox. 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa acid extract.

      Western Blot Analysis: 2 μg/mL (1:500) dilution of this antibody detected dimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2148290 2148290
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2395759 2395759
      Anti-dimethyl Histone H3 (Lys4), -2551186 2551186
      Anti-dimethyl Histone H3 (Lys4), -2583362 2583362
      Anti-dimethyl Histone H3 (Lys4), -2757107 2757107
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 1986174 1986174
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2063203 2063203
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2207239 2207239
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2320066 2320066
      Anti-dimethyl Histone H3 (Lys4), clone CMA303 - 2495668 2495668

      References

      Reference overviewPub Med ID
      Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.
      Konno, M; Koseki, J; Kawamoto, K; Nishida, N; Matsui, H; Dewi, DL; Ozaki, M; Noguchi, Y; Mimori, K; Gotoh, N; Tanuma, N; Shima, H; Doki, Y; Mori, M; Ishii, H
      PloS one  10  e0132789  2015

      Show Abstract
      26176628 26176628
      Decreased histone H2B monoubiquitination in malignant gastric carcinoma.
      Wang, ZJ; Yang, JL; Wang, YP; Lou, JY; Chen, J; Liu, C; Guo, LD
      World journal of gastroenterology  19  8099-107  2013

      Show Abstract
      24307806 24307806
      The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20.
      Lim, HJ; Dimova, NV; Tan, MK; Sigoillot, FD; King, RW; Shi, Y
      Molecular and cellular biology  33  4166-80  2013

      Show Abstract
      23979597 23979597
      LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes.
      Meier, K; Mathieu, EL; Finkernagel, F; Reuter, LM; Scharfe, M; Doehlemann, G; Jarek, M; Brehm, A
      PLoS genetics  8  e1002676  2012

      Show Abstract
      22570633 22570633
      Differential H3K4 methylation identifies developmentally poised hematopoietic genes.
      Orford, Keith, et al.
      Dev. Cell, 14: 798-809 (2008)  2008

      18477461 18477461
      Inhibition of lysine-specific demethylase 1 by polyamine analogues results in reexpression of aberrantly silenced genes.
      Huang, Yi, et al.
      Proc. Natl. Acad. Sci. U.S.A., 104: 8023-8 (2007)  2007

      17463086 17463086
      DNA methylation dictates histone H3K4 methylation.
      Okitsu, Cindy Yen and Hsieh, Chih-Lin
      Mol. Cell. Biol., 27: 2746-57 (2007)  2007

      17242185 17242185

      Technical Info

      Title
      Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303, 05-1338 ChIP-seq Analysis
      Anti-dimethyl-Histone H3 (Lys4) ChIP-seq Analysis

      Related Products & Applications

      Related Products

      Accessories

      Catalogue Number Description  
      07-030 Anti-dimethyl-Histone H3 (Lys4) Antibody Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies