09-454 | Anti-Dock2 Antibody

09-454
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Eq, PmWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-454
      DescriptionAnti-Dock2 Antibody
      Alternate Names
      • dedicator of cyto-kinesis 2
      • dedicator of cytokinesis 2
      Background InformationDock2 (Dedicator of cytokinesis 2), is a large protein involved in intracellular signaling networks and a member of the DOCK-A subfamily which function as activators of small G proteins. Dock2 specifically activates isoforms of the small G protein Rac and appears to be expressed specifically in leukocytes. These cells express both Rac1 and Rac2 and Dock2 has been shown to bind and promote nucleotide exchange on both of these isoforms. (Nishihara, 1999)
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse spleen lysate
      PresentationPurified in PBS containing 0.05% NaN3.
      Applications
      ApplicationWestern Blotting; Anti-Dock2 Antibody is an antibody against Dock2 for use in WB
      Immunohistchemistry (Frozen sections): A representative lot of this antibody dectected DOCK2 in mouse and human brain frozen tissue sections (Cimino, PJ et al. (2009). Am. J. Pathol. 175(4); 1622-1630)
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide near amino acid 1730, within the internal region of mouse DOCK2.
      EpitopeInternal region
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Specificity09-454 recognizes mouse DOCK2.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Horse
      • Primate
      Species Reactivity NoteMouse. Predicted to cross react with human, equine, and primate, based on 93% sequence homology (13/14 aa). The sequence is 85.7% homologous in rat. Reactivity with other species has not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.[supplied by OMIM]
      Gene Symbol
      • FLJ46592
      • KIAA0209
      Purification MethodPeptide affinity purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May participate in IL2 transcriptional activation via the activation of RAC2. Ref.1

      SUBUNIT: Interacts with RAC1 and RAC2. Interacts with CRKL and VAV. Interacts with CD3Z By similarity.

      SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane proteinBy similarity. Cytoplasm › cytoskeletonBy similarity. Note= Colocalizes with F-actin By similarity.

      TISSUE SPECIFICITY: Specifically expressed in hematopoietic cells. Ref.1

      DOMAIN: The DHR-2 domain probably mediates the GEF activity By similarity.

      SIMILARITY: Belongs to the DOCK family.

      Contains 1 DHR-1 (CZH-1) domain.

      Contains 1 DHR-2 (CZH-2) domain.

      Contains 1 SH3 domain.

      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western blot on mouse spleen lysate 0.5 μg/mL of Anti-DOCK2 at 1 mg/mL.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Dock2 2470739
      Anti-Dock2 - 2298432 2298432
      Anti-Dock2 - 2003022 2003022
      Anti-Dock2 - 2905374 2905374
      Anti-Dock2 - JBC1787316 JBC1787316
      Anti-Dock2 - NG1584936 NG1584936
      Anti-Dock2 - NG1842817 NG1842817
      Anti-Dock2 - NG1941997 NG1941997
      Anti-Dock2 -2489038 2489038
      Anti-Dock2 -2575907 2575907

      References

      Reference overviewPub Med ID
      Addendum: defective Dock2 expression in a subset of ASC-deficient mouse lines.
      Sirish K Ippagunta,R K Subbarao Malireddi,Patrick J Shaw,Geoffrey A Neale,Lieselotte Vande Walle,Yoshinori Fukui,Douglas R Green,Mohamed Lamkanfi,Thirumala-Devi Kanneganti
      Nature immunology  13  2012

      22905357 22905357
      DOCK2 is a microglial specific regulator of central nervous system innate immunity found in normal and Alzheimer's disease brain.
      Cimino, PJ; Sokal, I; Leverenz, J; Fukui, Y; Montine, TJ
      The American journal of pathology  175  1622-30  2009

      Show Abstract
      19729484 19729484
      Differential requirement for DOCK2 in migration of plasmacytoid dendritic cells versus myeloid dendritic cells.
      Gotoh, Kazuhito, et al.
      Blood, 111: 2973-6 (2008)  2008

      18198348 18198348
      A central role for DOCK2 during interstitial lymphocyte motility and sphingosine-1-phosphate-mediated egress.
      Nombela-Arrieta, César, et al.
      J. Exp. Med., 204: 497-510 (2007)  2007

      17325199 17325199
      Non-adherent cell-specific expression of DOCK2, a member of the human CDM-family proteins.
      Nishihara, H, et al.
      Biochim. Biophys. Acta, 1452: 179-87 (1999)  1999

      10559471 10559471