05-551 | Anti-E2F-3 Antibody, clone PG37

05-551
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-551
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-E2F-3 Antibody, clone PG37
      Background InformationE2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPBS, pH 7.4
      Applications
      ApplicationDetect E2F-3 with Anti-E2F-3 Antibody, clone PG37 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant full-length human E2F-3 protein
      Cloneclone PG37
      HostMouse
      SpecificityE2F-3
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
      Gene Symbol
      • E2F3
      • KIAA0075
      • E2F-3
      • MGC104598
      • DKFZp686C18211
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00716 # Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
      SIZE: 465 amino acids; 49162 Da
      SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: O00716 ## Belongs to the E2F/DP family.
      Molecular Weight58kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract or mouse 3T3 nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 2-8°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2392207 2392207
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2428491 2428491
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2441892 2441892
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2453107 2453107
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) 2938842
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2201364 2201364
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - 2275545 2275545
      Anti-E2F-3, clone PG37 (mouse monoclonal IgG2a) - NG1911119 NG1911119
      Anti-E2F-3, clone PG37 - 0702051752 0702051752
      Anti-E2F-3, clone PG37 - 19323 19323

      References

      Reference overviewApplicationPub Med ID
      Cellular transformation of mouse embryo fibroblasts in the absence of activator E2Fs.
      Gupta, T; Sáenz Robles, MT; Pipas, JM
      Journal of virology  89  5124-33  2015

      Show Abstract
      25717106 25717106
      Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143.
      Parker, JB; Yin, H; Vinckevicius, A; Chakravarti, D
      Cell reports  9  967-82  2014

      Show Abstract
      25437553 25437553
      E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3.
      Chen, D; Chen, Y; Forrest, D; Bremner, R
      Cell death and differentiation  20  931-40  2013

      Show Abstract
      23558950 23558950
      E2F3b over-expression in ovarian carcinomas and in BRCA1 Haploinsufficient fallopian tube epithelium.
      Na Lu Smith,Piri Welcsh,Joshua Z Press,Kathy J Agnew,Rochelle Garcia,Elizabeth M Swisher
      Genes, chromosomes & cancer  51  2012

      Show Abstract
      22887716 22887716
      MicroRNA-195 plays a tumor-suppressor role in human glioblastoma cells by targeting signaling pathways involved in cellular proliferation and invasion.
      Zhang, QQ; Xu, H; Huang, MB; Ma, LM; Huang, QJ; Yao, Q; Zhou, H; Qu, LH
      Neuro-oncology  14  278-87  2012

      Show Abstract
      Western Blotting22217655 22217655
      Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive.
      Coschi, CH; Martens, AL; Ritchie, K; Francis, SM; Chakrabarti, S; Berube, NG; Dick, FA
      Genes & development  24  1351-63  2010

      Show Abstract
      20551166 20551166
      Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer.
      Andersen, CL; Christensen, LL; Thorsen, K; Schepeler, T; Sørensen, FB; Verspaget, HW; Simon, R; Kruhøffer, M; Aaltonen, LA; Laurberg, S; Ørntoft, TF
      British journal of cancer  100  511-23  2009

      Show Abstract Full Text Article
      19156145 19156145
      RhoA regulates G1-S progression of gastric cancer cells by modulation of multiple INK4 family tumor suppressors.
      Zhang, S; Tang, Q; Xu, F; Xue, Y; Zhen, Z; Deng, Y; Liu, M; Chen, J; Liu, S; Qiu, M; Liao, Z; Li, Z; Luo, D; Shi, F; Zheng, Y; Bi, F
      Molecular cancer research : MCR  7  570-80  2009

      Show Abstract
      19372585 19372585
      The cyclin-dependent kinase inhibitor seliciclib (R-roscovitine; CYC202) decreases the expression of mitotic control genes and prevents entry into mitosis.
      Steven R Whittaker,Robert H Te Poele,Florence Chan,Spiros Linardopoulos,Michael I Walton,Michelle D Garrett,Paul Workman
      Cell cycle (Georgetown, Tex.)  6  2007

      Show Abstract
      18075315 18075315
      Amplification and overexpression of E2F3 in human bladder cancer.
      Feber, Andrew, et al.
      Oncogene, 23: 1627-30 (2004)  2004

      14716298 14716298

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies