05-312 | Anti-E2F-4 Antibody, clone GG22-2A6

05-312
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-312
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-E2F-4 Antibody, clone GG22-2A6
      References
      Product Information
      FormatPurified
      Presentation200μg of protein G purified mouse IgG2b in 200μl of 0.07 M Tris-glycine, pH 7.4, 0.135 M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      ApplicationDetect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenGST fusion to human E2F-4
      Cloneclone GG22-2A6
      HostMouse
      SpecificityE2F-4; does not recognize E2F-1, -2, -3, or -5
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
      Gene Symbol
      • E2F4
      • E2F-4
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16254 # Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
      SIZE: 413 amino acids; 43960 Da
      SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBBP4, RBL2, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.DEVELOPMENTAL STAGE: Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
      PTM: Differentially phosphorylated in vivo.
      SIMILARITY: SwissProt: Q16254 ## Belongs to the E2F/DP family.
      Molecular Weight55kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on non-stimulated A431 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-E2F-4, clone GG22-2A6 (mouse monoclonal IgG2b) - 2397089 2397089
      Anti-E2F-4, clone GG22-2A6 (mouse monoclonal IgG2b) - 2437348 2437348
      Anti-E2F-4, clone GG22-2A6 (mouse monoclonal IgG2b) 2857501
      Anti-E2F-4, clone GG22-2A6 (mouse monoclonal IgG2b) 2981050
      Anti-E2F-4, clone GG22-2A6 (mouse monoclonal IgG2b) - 2303947 2303947
      Anti-E2F-4, clone GG22-2A6 - 14387 14387
      Anti-E2F-4, clone GG22-2A6 - 28850 28850
      Anti-E2F-4, clone GG22-2A6 - 28850A 28850A
      Anti-E2F-4, clone GG22-2A6 - DAM1764386 DAM1764386

      References

      Reference overviewApplicationPub Med ID
      CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC.
      Borkham-Kamphorst, E; van Roeyen, CR; Van de Leur, E; Floege, J; Weiskirchen, R
      Journal of cell communication and signaling  6  11-25  2012

      Show Abstract
      21748432 21748432
      Dynamic loss of H2B ubiquitylation without corresponding changes in H3K4 trimethylation during myogenic differentiation.
      Vethantham, V; Yang, Y; Bowman, C; Asp, P; Lee, JH; Skalnik, DG; Dynlacht, BD
      Molecular and cellular biology  32  1044-55  2012

      Show Abstract
      22252316 22252316
      E2F-Rb complexes assemble and inhibit cdc25A transcription in cervical carcinoma cells following repression of human papillomavirus oncogene expression
      Wu, L., et al
      Mol Cell Biol, 20:7059-67 (2000)  2000

      Immunoblotting (Western)10982822 10982822
      Functional interaction between E2F-4 and p130: evidence for distinct mechanisms underlying growth suppression by different retinoblastoma protein family members.
      Vairo, G, et al.
      Genes Dev., 9: 869-81 (1995)  1995

      Show Abstract
      7705662 7705662
      E2F-4, a new member of the E2F transcription factor family, interacts with p107.
      Ginsberg, D, et al.
      Genes Dev., 8: 2665-79 (1994)  1994

      Show Abstract
      7958924 7958924

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies