05-1320 | Anti-EED Antibody, clone AA19

05-1320
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-1320
      DescriptionAnti-EED Antibody, clone AA19
      Alternate Names
      • WD protein associating with integrin cytoplasmic tails 1
      • embryonic ectoderm development
      Background InformationPolycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
      References
      Product Information
      FormatAscites
      Control
      • A431 Cell Lysate
      PresentationMouse monoclonal IgG2aκ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant fusion protein of full length murine EED tagged with MBP.
      CloneAA19
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes mouse EED, (~30-65 kDa).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Not tested in other species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HEED
      • WAIT-1
      • WAIT1
      • hEED
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. Ref.2 Ref.9 Ref.14 Ref.16 Ref.17 Ref.19 Ref.22 Ref.26

      SUBUNIT: Interacts with MLL By similarity. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. May interact with the MA protein of HIV-1.

      SUBCELLULAR LOCATION: Nucleus. Note= Transiently colocalizes with XIST at inactive X chromosomes. Ref.2 Ref.17 Ref.3 Ref.12 Ref.15

      SPECIFICITY: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. Ref.2 Ref.3 Ref.1 Ref.8 Ref.20

      Developmental stage Expression peaks at the G1/S phase boundary. Ref.14

      Induction Expression is induced by E2F1, E2F2 and E2F3. Ref.14

      SIMILARITY: Belongs to the WD repeat ESC family.

      Contains 7 WD repeats.

      Caution Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (Ref.18 and Ref.20), although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 (Ref.21).

      Molecular WeightRanges from 30-65 kDa (5-6 isoforms of EED are detected).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431cel lysatel.

      Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EED, clone AA19 - 2450188 2450188
      Anti-EED, clone AA19 - 2023175 2023175
      Anti-EED, clone AA19 - 2060503 2060503
      Anti-EED, clone AA19 - 2199689 2199689
      Anti-EED, clone AA19 - 2332588 2332588
      Anti-EED, clone AA19 - JBC1793811 JBC1793811
      Anti-EED, clone AA19 - NG1578250 NG1578250
      Anti-EED, clone AA19 - NG1624269 NG1624269
      Anti-EED, clone AA19 - NG1661338 NG1661338
      Anti-EED, clone AA19 - NG1689161 NG1689161

      References

      Reference overviewApplicationPub Med ID
      The central role of EED in the orchestration of polycomb group complexes.
      Cao, Q; Wang, X; Zhao, M; Yang, R; Malik, R; Qiao, Y; Poliakov, A; Yocum, AK; Li, Y; Chen, W; Cao, X; Jiang, X; Dahiya, A; Harris, C; Feng, FY; Kalantry, S; Qin, ZS; Dhanasekaran, SM; Chinnaiyan, AM
      Nature communications  5  3127  2014

      Show Abstract
      Immunoprecipitation24457600 24457600
      Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes.
      Reddington, JP; Perricone, SM; Nestor, CE; Reichmann, J; Youngson, NA; Suzuki, M; Reinhardt, D; Dunican, DS; Prendergast, JG; Mjoseng, H; Ramsahoye, BH; Whitelaw, E; Greally, JM; Adams, IR; Bickmore, WA; Meehan, RR
      Genome biology  14  R25  2013

      Show Abstract
      23531360 23531360
      Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells.
      Neri, F; Zippo, A; Krepelova, A; Cherubini, A; Rocchigiani, M; Oliviero, S
      Molecular and cellular biology  32  840-51  2012

      Show Abstract
      22184065 22184065
      Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.
      Zhang, Y; Yang, X; Gui, B; Xie, G; Zhang, D; Shang, Y; Liang, J
      The Journal of biological chemistry  286  42414-25  2011

      Show Abstract
      22009739 22009739
      The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
      Pasini, Diego, et al.
      Mol. Cell. Biol., 27: 3769-79 (2007)  2007

      Show Abstract
      17339329 17339329
      Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
      Bracken, Adrian P, et al.
      Genes Dev., 20: 1123-36 (2006)  2006

      Show Abstract
      16618801 16618801
      Polycomb group proteins in cell cycle progression and cancer.
      Pasini, Diego, et al.
      Cell Cycle, 3: 396-400 (2004)  2004

      14752272 14752272
      EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer.
      Bracken, Adrian P, et al.
      EMBO J., 22: 5323-35 (2003)  2003

      Show Abstract
      14532106 14532106

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies