05-235 | Anti-EF1α Antibody, clone CBP-KK1

05-235
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Bact, Ma, Yeast (S. cerevisiae)IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-235
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EF1α Antibody, clone CBP-KK1
      Alternate Names
      • CTCL tumor antigen
      • EF1a-like protein
      • Elongation factor 1 A-1
      • Elongation factor Tu
      • Leukocyte receptor cluster member 7
      • cervical cancer suppressor 3
      • elongation factor 1 alpha subunit
      • elongation factor 1-alpha
      • eukaryotic translation elongation factor 1 alpha 1
      • eukaryotic translation elongation factor 1 alpha 1-like 14
      • glucocorticoid receptor AF-1 specific elongation factor
      • leukocyte receptor cluster (LRC) member 7
      • prostate tumor-inducing protein 1
      • translation elongation factor 1 alpha 1-like 14
      Background InformationHuman eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
      The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate.
        Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.
      Biological Information
      ImmunogenCrude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.
      CloneCBP-KK1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      • Mammals
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
      Gene Symbol
      • EEF1A1
      • eEF1A-1
      • FLJ25721
      • EF-1-alpha-1
      • EEF-1
      • HNGC:16303
      • ALPHA
      • CCS-3
      • PTI1
      • MGC131894
      • EEF1A
      • CCS3
      • MGC102687
      • EF-Tu
      • MGC16224
      • GRAF-1EF
      • LENG7
      • EF1A
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68104 # This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
      SIZE: 462 amino acids; 50141 Da
      SUBUNIT: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with XPO5.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
      SIMILARITY: SwissProt: P68104 ## Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
      Molecular Weight53 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EF1#945;, clone CBP-KK1 - 2120135 2120135
      Anti-EF1#945;, clone CBP-KK1 - DAM1821043 DAM1821043
      Anti-EF1, clone CBP-KK1 - 2044186 2044186
      Anti-EF1, clone CBP-KK1 - 2193144 2193144
      Anti-EF1, clone CBP-KK1 - 2363001 2363001
      Anti-EF1, clone CBP-KK1 - DAM1394752 DAM1394752
      Anti-EF1, clone CBP-KK1 - DAM1493396 DAM1493396
      Anti-EF1, clone CBP-KK1 - DAM1690617 DAM1690617
      Anti-EF1, clone CBP-KK1 - DAM1745276 DAM1745276
      Anti-EF1, clone CBP-KK1 - JBC1863400 JBC1863400

      References

      Reference overviewApplicationPub Med ID
      Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances.
      Mani, J; Desy, S; Niemann, M; Chanfon, A; Oeljeklaus, S; Pusnik, M; Schmidt, O; Gerbeth, C; Meisinger, C; Warscheid, B; Schneider, A
      Nature communications  6  6646  2015

      Show Abstract
      25808593 25808593
      ADAM9 up-regulates N-cadherin via miR-218 suppression in lung adenocarcinoma cells.
      Sher, YP; Wang, LJ; Chuang, LL; Tsai, MH; Kuo, TT; Huang, CC; Chuang, EY; Lai, LC
      PloS one  9  e94065  2014

      Show Abstract
      Western Blotting24705471 24705471
      Translational control in the stress adaptive response of cancer cells: a novel role for the heat shock protein TRAP1.
      Matassa, DS; Amoroso, MR; Agliarulo, I; Maddalena, F; Sisinni, L; Paladino, S; Romano, S; Romano, MF; Sagar, V; Loreni, F; Landriscina, M; Esposito, F
      Cell death & disease  4  e851  2013

      Show Abstract
      24113185 24113185
      Co-infection with the friend retrovirus and mouse scrapie does not alter prion disease pathogenesis in susceptible mice.
      Leblanc, P; Hasenkrug, K; Ward, A; Myers, L; Messer, RJ; Alais, S; Timmes, A; Priola, SA; Priola, S
      PloS one  7  e30872  2012

      Show Abstract
      22295118 22295118
      Sulpiride, but not SCH23390, modifies cocaine-induced conditioned place preference and expression of tyrosine hydroxylase and elongation factor 1α in zebrafish.
      Darland, T; Mauch, JT; Meier, EM; Hagan, SJ; Dowling, JE; Darland, DC
      Pharmacology, biochemistry, and behavior  103  157-67  2012

      Show Abstract
      Western Blotting22910534 22910534
      Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.
      Saikia, M; Krokowski, D; Guan, BJ; Ivanov, P; Parisien, M; Hu, GF; Anderson, P; Pan, T; Hatzoglou, M
      The Journal of biological chemistry  287  42708-25  2012

      Show Abstract
      Immunoprecipitation23086926 23086926
      A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
      Yun-Hong, Y; Chih-Fan, C; Chia-Wei, C; Yen-Chung, C
      Molecular & cellular proteomics : MCP  10  M110.007138  2011

      Show Abstract
      21715321 21715321
      Glutamate regulates eEF1A phosphorylation and ribosomal transit time in Bergmann glial cells.
      Barrera I, Flores-Méndez M, Hernández-Kelly LC, Cid L, Huerta M, Zinker S, López-Bayghen E, Aguilera J, Ortega A
      Neurochem Int  57  795-803. Epub 2010 Sep 9.  2010

      Show Abstract
      20817065 20817065
      Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
      Broytman, O; Westmark, PR; Gurel, Z; Malter, JS
      Neurobiology of aging  30  1962-74  2009

      Show Abstract
      18378046 18378046
      Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and stimulates Il1a transcription in rat Sertoli cells.
      Tash, JS; Chakrasali, R; Jakkaraj, SR; Hughes, J; Smith, SK; Hornbaker, K; Heckert, LL; Ozturk, SB; Hadden, MK; Kinzy, TG; Blagg, BS; Georg, GI
      Biology of reproduction  78  1139-52  2008

      Show Abstract
      18218611 18218611

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies