04-338 | Anti-EGFR Antibody, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IP, WBRbPurifiedMonoclonal Antibody
      Catalogue Number04-338
      Replaces04-337; 05-1128
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EGFR Antibody, rabbit monoclonal
      Alternate Names
      • Epidermal growth factor receptor
      • ERBB
      • ERBB1
      Product Information
      • POSITIVE: A431 cells.
      PresentationRabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-EGFR Antibody, rabbit detects level of EGFR & has been published & validated for use in FC, IF, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot: 1:10,000 on lysates from A431 cells.
      Immunoprecipitation: 1:30.
      Flow Cytometry: 1:10. Permeablization of cells required.
      Immunofluorescence: 1:50. Paraformaldehyde fixation recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the C-terminal region of human EGF Receptor (EGFR).
      SpecificityRecognizes the C-terminal domain of EGFR and does not cross-react with any other ErbB family member.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight170 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EGFR, clone E235 2465141
      Anti-EGFR, clone E235 (Rabbit Monoclonal - 2064282 2064282
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - 2197104 2197104
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - 2297143 2297143
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - DAM1682188 DAM1682188
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - DAM1713254 DAM1713254
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - DAM1780427 DAM1780427
      Anti-EGFR, clone E235 (Rabbit Monoclonal) - NG1944013 NG1944013
      Anti-EGFR, clone E235 - 2519203 2519203
      Anti-EGFR, rabbit monoclonal - 0611045575 0611045575


      Reference overviewApplicationPub Med ID
      The failure in the stabilization of glioblastoma-derived cell lines: spontaneous in vitro senescence as the main culprit.
      Stoczynska-Fidelus, E; Piaskowski, S; Bienkowski, M; Banaszczyk, M; Hulas-Bigoszewska, K; Winiecka-Klimek, M; Radomiak-Zaluska, A; Och, W; Borowiec, M; Zieba, J; Treda, C; Rieske, P
      PloS one  9  e87136  2014

      Show Abstract
      24498027 24498027
      EGF receptor and COX-1/COX-2 enzyme proteins as related to corresponding mRNAs in human per-operative biopsies of colorectal cancer.
      Asting, AG; Farivar, A; Iresjö, BM; Svensson, H; Gustavsson, B; Lundholm, K
      BMC cancer  13  511  2013

      Show Abstract
      Western Blotting24171795 24171795
      The MARCH family E3 ubiquitin ligase K5 alters monocyte metabolism and proliferation through receptor tyrosine kinase modulation.
      Karki, R; Lang, SM; Means, RE
      PLoS pathogens  7  e1001331  2011

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)21490960 21490960
      Integration of growth factor, extracellular matrix, and retinoid signals during bronchial epithelial cell differentiation.
      Moghal, N and Neel, B G
      Mol. Cell. Biol., 18: 6666-78 (1998)  1998

      Show Abstract
      Neutralization Assay9774681 9774681

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