05-1047 | Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2

05-1047
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1047
      Replaces04-338
      DescriptionAnti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2
      Alternate Names
      • Receptor tyrosine-protein kinase ErbB-1
      • avian erythroblastic leukemia viral (v-erb-b) oncogene
        homolog
      • cell growth inhibiting protein 40
      • cell proliferation-inducing protein 61
      • epidermal growth factor receptor
      • epidermal growth factor receptor (avian erythroblastic
        leukemia viral (v-erb-b) oncogene homolog)
      • epidermal growth factor receptor (erythroblastic leukemia
        viral (v-erb-b) oncogene homolog, avian)
      Background InformationThe epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs. These are Y992, Y1045, Y1068, Y1148 and Y1173. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. Such proteins modulate phenotypes such as cell migration, adhesion, and proliferation. The kinase domain of EGFR can also cross-phosphorylate tyrosine residues of other receptors it is aggregated with, and can itself be activated in that manner.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect EGFR (cytoplasmic domain) using this Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 validated for use in IC, IH, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis:
      A431 cells were grown, fixed, permeablized, and stained with anti-EGFR, clone 8G6.2

      Confocal Immunocytochemistry Analysis:
      A431 cells were grown, fixed, permeablized, and stained with anti-EGFR, clone 8G6.2

      Immunoprecipitation:
      100 μg of A431 whole cell lysate was lysed in RIPA buffer and immunoprecipitated (IP) with 5 μg of Anti-EGFR, cytoplasmic domain, clone 8G6.2

      Immunohistochemistry Analysis:
      Tissue was stained with anti-EGFR, cytoplasmic domain, clone 8G6.2 at a 1:100 dilution and IHC-Select Detection with HRP-DAB reagents.


      Biological Information
      ImmunogenRecombinant protein encoding the cytoplasmic domain (C-terminal) of human EGF Receptor.
      EpitopeCytoplasmic Domain
      Clone8G6.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects the cytoplasmic domain of EGFR.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteEvaluated by western blot on A431 cell lysate
      Western Blot Analysis:
      1:2,000 dilution of this antibody was used to detect EGFR in A431 cell lysate.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on A431 cell lysate
      Western Blot Analysis:
      1:2,000 dilution of this antibody was used to detect EGFR in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EGFR (cytoplasmic domain), -2551831 2551831
      Anti-EGFR (cytoplasmic domain), -2794925 2794925
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 2488839
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2151860 2151860
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2388924 2388924
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 1975107 1975107
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2049285 2049285
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2179366 2179366
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2199722 2199722
      Anti-EGFR (cytoplasmic domain), clone 8G6.2 - 2262726 2262726

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies