05-484 | Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-484
      Replaces04-337; 04-341
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3
      Product Information
      Presentation100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
      ApplicationDetect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
      Cloneclone 20G3
      SpecificityThe motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight175kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EGF-R (non-phospho-Y1173) (mouse monoclonal IgG1#954;) - 2115688 2115688
      Anti-EGF-R (non-phospho-Y1173) (mouse monoclonal IgG1#954;) - 2121961 2121961
      Anti-EGF-R (non-phospho-Y1173) (mouse monoclonal IgG1k) - 2316793 2316793
      Anti-EGF-R (non-phospho-Y1173) - 2427480 2427480
      Anti-EGF-R (non-phospho-Y1173) - 2152220 2152220
      Anti-EGF-R (non-phospho-Y1173) - JBC1874347 JBC1874347
      Anti-EGFR (non-phospho-Tyr1173), clone 20G3 - 16984 16984
      Anti-EGFR (non-phospho-Tyr1173), clone 20G3 - 19340 19340
      Anti-EGFR (non-phospho-Tyr1173), clone 20G3 - 20783 20783
      Anti-EGFR (non-phospho-Tyr1173), clone 20G3 - 22762 22762


      Reference overviewApplicationPub Med ID
      Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice.
      Siggs, OM; Grieve, A; Xu, H; Bambrough, P; Christova, Y; Freeman, M
      Biology open  3  1151-7  2014

      Show Abstract
      25395669 25395669
      Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells.
      Chen, G; Kronenberger, P; Teugels, E; De Grève, J
      Biological procedures online  13  1  2011

      Show Abstract Full Text Article
      Western Blotting21369532 21369532
      Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
      Sorkin, A, et al.
      J. Biol. Chem., 267: 8672-8 (1992)  1992

      Show Abstract
      1314835 1314835
      Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
      Bertics, P J, et al.
      J. Biol. Chem., 263: 3610-7 (1988)  1988

      3346210 3346210

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