07-689 | Anti-EZH2 Antibody

200 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ChIP-seqRbSerumPolyclonal Antibody
      Catalogue Number07-689
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EZH2 Antibody
      Alternate Names
      • KMT6
      • Enx1
      • Enhancer of Zeste Homologue 2
      Background InformationPolycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.
      Product Information
      • K562 cell lysate, C2C12 cell lysate
      ApplicationThis Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenHis-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2)
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • EZH2
      • MGC9169
      • ENX-1
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15910 # May be involved in the regulation of gene transcription and chromatin structure.
      SIZE: 746 amino acids; 85363 Da
      SUBUNIT: Binds ATRX via the SET domain (Probable).
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Expressed in many tissues.
      SIMILARITY: SwissProt: Q15910 ## Belongs to the EZ family. & Contains 1 SET domain.
      Molecular Weight95-100kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable for 2 years at 2-8°C from date of shipment
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EZH2 2477979
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - 1982631 1982631
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1578238 DAM1578238
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1588132 DAM1588132
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1598793 DAM1598793
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1614932 DAM1614932
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1632086 DAM1632086
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1731502 DAM1731502
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1795387 DAM1795387
      Anti-EZH2 (Enhancer of Zeste Homologue 2) - DAM1834941 DAM1834941


      Reference overviewApplicationSpeciesPub Med ID
      Live cell imaging of the nascent inactive X chromosome during the early differentiation process of naive ES cells towards epiblast stem cells.
      Guyochin, A; Maenner, S; Chu, ET; Hentati, A; Attia, M; Avner, P; Clerc, P
      PloS one  9  e116109  2014

      Show Abstract
      Immunofluorescence25546018 25546018
      Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.
      Ratovitski, EA
      Cell cycle (Georgetown, Tex.)  13  749-61  2014

      Show Abstract
      Western Blotting, Immunoprecipitation24394434 24394434
      Disruption of the MYC-miRNA-EZH2 loop to suppress aggressive B-cell lymphoma survival and clonogenicity.
      Zhao, X; Lwin, T; Zhang, X; Huang, A; Wang, J; Marquez, VE; Chen-Kiang, S; Dalton, WS; Sotomayor, E; Tao, J
      Leukemia  27  2341-50  2013

      Show Abstract
      23538750 23538750
      A pseudogene long-noncoding-RNA network regulates PTEN transcription and translation in human cells.
      Johnsson, P; Ackley, A; Vidarsdottir, L; Lui, WO; Corcoran, M; Grandér, D; Morris, KV
      Nature structural & molecular biology  20  440-6  2013

      Show Abstract
      23435381 23435381
      Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A.
      Xu, J; Bauer, DE; Kerenyi, MA; Vo, TD; Hou, S; Hsu, YJ; Yao, H; Trowbridge, JJ; Mandel, G; Orkin, SH
      Proceedings of the National Academy of Sciences of the United States of America  110  6518-23  2013

      Show Abstract
      Human23576758 23576758
      Heterogeneity of chromatin modifications in testicular spermatocytic seminoma point toward an epigenetically unstable phenotype.
      Dina G Kristensen,Olga Mlynarska,John E Nielsen,Grete K Jacobsen,Ewa Rajpert-De Meyts,Kristian Almstrup
      Cancer genetics  205  2012

      Show Abstract
      22819380 22819380
      Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1.
      Hong, GM; Bain, LJ
      Toxicology and applied pharmacology  260  250-9  2012

      Show Abstract
      22426358 22426358
      PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.
      Illingworth, RS; Botting, CH; Grimes, GR; Bickmore, WA; Eskeland, R
      PloS one  7  e34848  2012

      Show Abstract
      22496869 22496869
      ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression.
      Abdel-Wahab, O; Adli, M; LaFave, LM; Gao, J; Hricik, T; Shih, AH; Pandey, S; Patel, JP; Chung, YR; Koche, R; Perna, F; Zhao, X; Taylor, JE; Park, CY; Carroll, M; Melnick, A; Nimer, SD; Jaffe, JD; Aifantis, I; Bernstein, BE; Levine, RL
      Cancer cell  22  180-93  2012

      Show Abstract
      22897849 22897849
      ALDH1A1 is a novel EZH2 target gene in epithelial ovarian cancer identified by genome-wide approaches.
      Li, H; Bitler, BG; Vathipadiekal, V; Maradeo, ME; Slifker, M; Creasy, CL; Tummino, PJ; Cairns, P; Birrer, MJ; Zhang, R
      Cancer prevention research (Philadelphia, Pa.)  5  484-91  2012

      Show Abstract
      22144423 22144423

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Life Science Research > Antibodies and Assays > Primary Antibodies