07-1583 | Anti-EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal

07-1583
100 µL  
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      Overview

      Replacement Information
      Replacement Information04-1047 is the recommended replacement for Discontinued catalog number 07-1583

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, XnWB, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1583
      DescriptionAnti-EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal
      Alternate Names
      • Enhancer of zeste homolog 2
      • Lysine N-methyltransferase 6
      • enhancer of zeste 2
      • enhancer of zeste homolog 2
      • ENX-1
      Background InformationPolycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate.
      PresentationAffinity purified from serum. Supplied at 0.57 mg/ml in PBS containing 0.05% sodium azide.
      Applications
      ApplicationThis Anti-EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal is validated for use in WB, ELISA for the detection of EZH2 (Enhancer of Zeste Homologue 2).
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesWestern Blot Analysis:
      A 1:1000 dilution of this lot detected EZH2 in HeLa cell lysate (Figure A).

      ELISA
      The antibody has also been shown to work in direct peptide ELISA (data not shown).
      Biological Information
      ImmunogenImmunogen was a KLH-conjugated synthetic peptide corresponding to a.a. 260-277 (CTPNIDGPNAKSVQREQS) of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2).
      Epitopea.a. 260-277
      HostRabbit
      SpecificityRecognizes EZH2, Mr ~100 kDa.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Xenopus
      Species Reactivity NoteHuman. Expected to cross-react with mouse, rat, and Xenopus (bullfrog) based on 100% sequence identity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
      Gene Symbol
      • EZH2
      • KMT6
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine.
      SUBUNIT: Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2.
      INTERACTION:
      P26358:DNMT1; NbExp=3; IntAct=EBI-530054, EBI-719459;
      Q9Y6K1:DNMT3A; NbExp=3; IntAct=EBI-530054, EBI-923653;
      Q9UBC3:DNMT3B; NbExp=3; IntAct=EBI-530054, EBI-80125;
      P10276:RARA; NbExp=2; IntAct=EBI-530054, EBI-413374;

      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
      DEVELOPMENTAL STAGE: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
      INDUCTION: Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of TP53/p53.
      PTM: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
      SIMILARITY: Belongs to the histone-lysine methyltransferase family. EZ subfamily.
      SIMILARITY: Contains 1 SET domain.
      CAUTION: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (PubMed:15099518 and PubMed:15684044), although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 (PubMed:16431907).
      Molecular Weight85-100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: To maximize recovery, centrifuge briefly prior to removing the cap. Avoid freeze/thawing the product, which can damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies