04-226 | Anti-Elk1 Antibody, (CT), clone E277, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-226
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Elk1 Antibody, (CT), clone E277, rabbit monoclonal
      Background InformationELK1 is a transcription factor and a member of the Ets family of transcription factors. Elk1 is a downstream transcription factor of the Ras-Raf-MEK-Erk (canonical MAPK) pathway.
      Product Information
      FormatCulture Supernatant
      • A431 whole cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer contaiing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationAnti-Elk1 Antibody, (C-term), clone E277, rabbit is an antibody against Elk1 for use in IC, IH, IH(P), IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: 1:50 dilution from a previous lot was used on immunohistochemistry.

      Immunocytochemistry: 1:100 dilution from a previous lot was used on immunocytochemistry.

      Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
      Biological Information
      ImmunogenKLH conjugated linear peptide of Elk-1.
      SpecificityThis antibody recognizes Elk-1 at the C-Terminus. The antibody does not cross-react with other ETS family members.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Not expected to react with mouse and rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
      UniProt Number
      UniProt SummaryFunction: Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
      Subunit structure: Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Ref.12
      Subcellular location: Nucleus.
      Tissue specificity: Lung and testis.
      Post-translational modification: Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
      On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Ref.7
      Sequence similarities: Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.

      Molecular Weight47-60 kDa Molecular weight varies depending on modifications.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 whole cell lysate.

      Western Blot Analysis:
      A 1:500 dilution of this lot was used to detect Elk-1 on A431 whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information