CBL1358 | Anti-Endoglin Antibody, Extracellular, clone MJ7/18

CBL1358
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IP, WB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1358
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Endoglin Antibody, Extracellular, clone MJ7/18
      Alternate Names
      • CD105
      • Ancillary TGF-beta Receptor
      Background InformationEndoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: mouse vascular endothelial cells
      PresentationProtein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives.
      Applications
      ApplicationThis Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesFlow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.

      Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.

      Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.

      Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenInflamed mouse skin (Ge, AZ et al, 1994).
      CloneMJ7/18
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificitySpecifically recognizes mouse CD105, known as endoglin.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryEndoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity. Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • ENG
      • ORW
      • CD105
      • HHT1
      • END
      • FLJ41744
      • ORW1
      • endoglin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17813 # Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
      SIZE: 658 amino acids; 70578 Da
      SUBUNIT: Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGF-beta receptors I and/or II. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
      DISEASE: SwissProt: P17813 # Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2135075 2135075
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2446708 2446708
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 1990922 1990922
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2101951 2101951
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2211007 2211007
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2273801 2273801
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2310333 2310333
      RAT ANTI-MOUSE ENDOGLIN [CD105] MONOCLONAL ANTIBODY - 2510167 2510167

      References

      Reference overviewPub Med ID
      Nanoparticle-mediated drug delivery to tumor vasculature suppresses metastasis.
      Murphy, EA; Majeti, BK; Barnes, LA; Makale, M; Weis, SM; Lutu-Fuga, K; Wrasidlo, W; Cheresh, DA
      Proceedings of the National Academy of Sciences of the United States of America  105  9343-8  2008

      Show Abstract
      18607000 18607000
      Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand
      Ge, AZ and Butcher, EC.
      Gene, 138:201-206 (1994)  1994

      8125301 8125301

      Data Sheet

      Title
      RAT ANTI-MOUSE ENDOGLIN [CD105]

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies