CBL418F | Anti-Endoglin Antibody, clone 8E11, FITC conjugated

CBL418F
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IFMFITCMonoclonal Antibody
      Description
      Catalogue NumberCBL418F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Endoglin Antibody, clone 8E11, FITC conjugated
      Alternate Names
      • CD105
      • ancillary TGF-beta Receptor
      References
      Product Information
      FormatFITC
      PresentationThe conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.
      Applications
      ApplicationAnti-Endoglin Antibody, clone 8E11, FITC conjugated detects level of Endoglin & has been published & validated for use in FC & IF.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      Application NotesStudy of monocyte differentiation into macrophages and endoglin up-regulation.

      Studies in cellular adhesion of circulating blood cells.

      Lysis of CD105+ cells in the presence of complement.

      Identification of CD105 positive cells on frozen tissue sections.

      Flow cytometry: use 10μL of conjugate per test

      We recommend that each laboratory determine an optimum working titre for use in its particular application.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPMA-induced U-937 cells. U-937 is an human lymphoma cell line that expresses markers and properties of monocytes.
      Clone8E11
      HostMouse
      SpecificityEndoglin is an endothelial homodimeric membrane antigen containing the tripeptide sequence RGD, which is a recognition motif for adhesion receptors of the integrin family. It has been proposed that endoglin is a TGF-beta receptor. Endoglin is a glycoprotein present on endothelial cells of capillaries, arterioles and venules in a variety of tissues and at low levels on acute lymphoblastic and myelocytic leukaemia cells.

      FUSION PARTNER: NS1 myeloma cell line.
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryEndoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity. Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • ENG
      • ORW
      • CD105
      • HHT1
      • END
      • FLJ41744
      • ORW1
      • endoglin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17813 # Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
      SIZE: 658 amino acids; 70578 Da
      SUBUNIT: Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGF-beta receptors I and/or II. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
      DISEASE: SwissProt: P17813 # Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CD105 FITC CONJUGATED MONOCLONAL ANTIBODY - 2388950 2388950
      MOUSE ANTI-HUMAN CD105 FITC CONJUGATED MONOCLONAL ANTIBODY - 2455688 2455688
      MOUSE ANTI-HUMAN CD105 FITC CONJUGATED MONOCLONAL ANTIBODY - 2051865 2051865
      MOUSE ANTI-HUMAN CD105 FITC CONJUGATED MONOCLONAL ANTIBODY - 2199605 2199605
      MOUSE ANTI-HUMAN CD105 FITC CONJUGATED MONOCLONAL ANTIBODY - 2325761 2325761

      References

      Reference overviewPub Med ID
      Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells.
      Cervelló, I; Gil-Sanchis, C; Mas, A; Delgado-Rosas, F; Martínez-Conejero, JA; Galán, A; Martínez-Romero, A; Martínez, S; Navarro, I; Ferro, J; Horcajadas, JA; Esteban, FJ; O'Connor, JE; Pellicer, A; Simón, C
      PloS one  5  e10964  2010

      Show Abstract Full Text Article
      20585575 20585575

      Data Sheet

      Title
      Anti-Endoglin, Clone 8E11, FITC Conjugated - Data Sheet