CBL419 | Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

CBL419
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL419
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Eosinophil Major Basic Protein Antibody, clone BMK13
      Alternate Names
      • EMBP
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
      Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
      ELISA development for MBP estimation.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      CloneBMK13
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityCBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.
      Gene Symbol
      • PRG2
      • MBP
      • BMPG
      • MGC14537
      Non-Reactive Species
      • Horse
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13727 # Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
      SIZE: 222 amino acids; 25232 Da
      SUBUNIT: In pregnancy serum, the proform exists as a disulfide- linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
      SUBCELLULAR LOCATION: Note=The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core).
      TISSUE SPECIFICITY: High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.DEVELOPMENTAL STAGE: Levels of the proform increase in serum and placenta during pregnancy.
      SIMILARITY: SwissProt: P13727 ## Contains 1 C-type lectin domain.
      MISCELLANEOUS: Binds heparin. Does not bind calcium.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2120959 2120959
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2356100 2356100
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) -2533838 2533838
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) -2569778 2569778
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) -2585780 2585780
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) -2721680 2721680
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) -2769027 2769027
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY 2898908
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2069950 2069950
      MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2191964 2191964

      References

      Reference overviewPub Med ID
      Age-related changes in proliferation, the numbers of mast cells, eosinophils, and cd45-positive cells in human dermis.
      Gunin AG, Kornilova NK, Vasilieva OV, Petrov VV
      The journals of gerontology Series A, Biological sciences and medical sciences  66  385-92. Epub 2010 Nov 24.  2011

      Show Abstract
      21106704 21106704

      Data Sheet

      Title
      Anti-Eosinophil Major Basic Protein, clone BMK13 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies