CBL251 | Anti-Epithelial Specific Antigen Antibody, clone VU-1D9

CBL251
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL251
      DescriptionAnti-Epithelial Specific Antigen Antibody, clone VU-1D9
      Alternate Names
      • Epithelial cell adhesion molecule
      • Ep-CAM
      • Adenocarcinoma-associated antigen
      • Cell surface glycoprotein Trop-1
      • Epithelial cell surface antigen
      • Epithelial glycoprotein
      • EGP
      • Epithelial glycoprotein 314
      • EGP314
      • hEGP314
      • KS 1/4 antigen
      • KSA
      • Major gastrointestinal tumor-associated protein GA733-2
      • Tumor-associated calcium signal transducer 1
      • CD326
      Background InformationEpithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.
      References
      Product Information
      FormatPurified
      Control
      • MCF7 cell lyasate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.
      Biological Information
      ImmunogenNCI-H69 whole cells
      CloneVU-1D9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EPCAM
      • GA733-2
      • M1S2
      • M4S1
      • MIC18
      • TACSTD1
      • TROP1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lyate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Epithelial Specific Antigen, clone VU-1D9 - 2148197 2148197
      Anti-Epithelial Specific Antigen, clone VU-1D9 - 2453167 2453167
      Anti-Epithelial Specific Antigen, clone VU-1D9 -2585874 2585874
      Anti-Epithelial Specific Antigen, clone VU-1D9 -2586511 2586511
      Anti-Epithelial Specific Antigen, clone VU-1D9 -2728366 2728366
      Anti-Epithelial Specific Antigen, clone VU-1D9 -2831907 2831907
      Anti-Epithelial Specific Antigen, clone VU-1D9 Monoclonal Antibody 2886497

      Data Sheet

      Title
      Anti-Epithelial Specific Antigen, clone VU-1D9 - Data Sheet

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      Alternative Packsize

      Catalogue Number Description  
      CBL251-KC Anti-Epithelial Specific Antigen, c Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies