04-227 | Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal

04-227
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IH(P)RbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-227
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
      Alternate Names
      • Estradiol receptor
      • Nuclear receptor subfamily 3 group A member 1
      • estrogen receptor 1
      • estrogen receptor alpha
      • oestrogen receptor
      • steroid hormone receptor
      Background InformationEstrogen Receptor α (ER alpha) is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • MCF-7 cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Estrogen Receptor α Antibody, clone E115 is validated for use in WB, IC, IH(P) for the detection of Estrogen Receptor α.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry:

      Immunocytochemistry:
      1:100 dilution of a previous lot was used on immunocytochemistry.
      Biological Information
      ImmunogenSynthetic peptide directed against human Estrogen Receptor α.
      CloneE115
      HostRabbit
      SpecificityThis antibody recognizes Estrogen Receptor α.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse and rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for
      hormone binding, DNA binding, and activation of transcription. The
      protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast
      cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq].
      Gene Symbol
      • ER
      • ER-alpha
      • ESR
      • ESRA
      • Era
      • NR3A1
      • DKFZp686N23123
      • OTTHUMP00000017718
      • OTTHUMP00000017719
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Molecular Weight67 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on MCF-7 cell lysate.

      Western Blot Analysis:
      A 1:250-1:500 dilution of this lot was used to detect Estrogen Receptor  in MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Estrogen Receptor #945;, clone E115 - 2145048 2145048
      Anti-Estrogen Receptor #945;,clone E115 - NG1820199 NG1820199
      Anti-Estrogen Receptor a, clone E115 - 1983664 1983664
      Anti-Estrogen Receptor a, clone E115 - 2024178 2024178
      Anti-Estrogen Receptor a, clone E115 - 2107206 2107206
      Anti-Estrogen Receptor a, clone E115 - 2270854 2270854
      Anti-Estrogen Receptor a, clone E115 - 2294327 2294327
      Anti-Estrogen Receptor a, clone E115 - 2361788 2361788
      Anti-Estrogen Receptor a, clone E115 - DAM1647894 DAM1647894
      Anti-Estrogen Receptor a, clone E115 - DAM1682189 DAM1682189

      References

      Reference overviewApplicationPub Med ID
      Alterations in ubiquitin ligase Siah-2 and its corepressor N-CoR after P-MAPA immunotherapy and anti-androgen therapy: new therapeutic opportunities for non-muscle invasive bladder cancer.
      Garcia, PV; Apolinário, LM; Böckelmann, PK; da Silva Nunes, I; Duran, N; Fávaro, WJ
      International journal of clinical and experimental pathology  8  4427-43  2015

      Show Abstract
      26191134 26191134
      Folate Deficiency Could Restrain Decidual Angiogenesis in Pregnant Mice.
      Li, Y; Gao, R; Liu, X; Chen, X; Liao, X; Geng, Y; Ding, Y; Wang, Y; He, J
      Nutrients  7  6425-45  2015

      Show Abstract
      26247969 26247969
      Tyrosine phosphatase Shp2 mediates the estrogen biological action in breast cancer via interaction with the estrogen extranuclear receptor.
      Li, J; Kang, Y; Wei, L; Liu, W; Tian, Y; Chen, B; Lin, X; Li, Y; Feng, GS; Lu, Z
      PloS one  9  e102847  2014

      Show Abstract
      Western Blotting25048202 25048202
      Sex-dependent differences in intestinal tumorigenesis induced in Apc1638N/+ mice by exposure to γ rays.
      Trani, D; Moon, BH; Kallakury, B; Hartmann, DP; Datta, K; Fornace, AJ
      International journal of radiation oncology, biology, physics  85  223-9  2013

      Show Abstract
      22503525 22503525
      Exposure to ionizing radiation causes long-term increase in serum estradiol and activation of PI3K-Akt signaling pathway in mouse mammary gland.
      Suman, S; Johnson, MD; Fornace, AJ; Datta, K
      International journal of radiation oncology, biology, physics  84  500-7  2012

      Show Abstract
      22381906 22381906
      MGMT inhibition restores ERα functional sensitivity to antiestrogen therapy.
      Bobustuc, GC; Smith, JS; Maddipatla, S; Jeudy, S; Limaye, A; Isley, B; Caparas, ML; Constantino, SM; Shah, N; Baker, CH; Srivenugopal, KS; Baidas, S; Konduri, SD
      Molecular medicine (Cambridge, Mass.)  18  913-29  2012

      Show Abstract
      22549111 22549111

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      Categories

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