07-130 | Anti-Ezrin Antibody

07-130
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-130
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ezrin Antibody
      OverviewEzrin is also referred to as Villin-2 and Cytovillin
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Ezrin Antibody is validated for use in WB, IH for the detection of Ezrin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Immunogenpeptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG)
      HostRabbit
      SpecificityEzrin
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
      Gene Symbol
      • VIL2
      • Ezrin
      • CVL
      • Cytovillin
      • FLJ26216
      • CVIL
      • Villin-2
      • ezrin
      • MGC1584
      • cytovillin
      • p81
      • DKFZp762H157
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15311 # Probably involved in connections of major cytoskeletal structures to the plasma membrane.
      SIZE: 586 amino acids; 69413 Da
      SUBUNIT: Interacts with MPP5 (By similarity). Interacts with SLC9A3R1 and PACE1.
      SUBCELLULAR LOCATION: Membrane. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).
      TISSUE SPECIFICITY: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.DEVELOPMENTAL STAGE: Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
      PTM: Phosphorylated by tyrosine-protein kinases.
      SIMILARITY: SwissProt: P15311 ## Contains 1 FERM domain.
      Molecular Weight81kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells or a mouse brain lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ezrin (rabbit polyclonal IgG) - 2462878 2462878
      Anti-Ezrin (rabbit polyclonal IgG) 2964138
      Anti-Ezrin (rabbit polyclonal IgG) - 1984842 1984842
      Anti-Ezrin (rabbit polyclonal IgG) - 2028731 2028731
      Anti-Ezrin (rabbit polyclonal IgG) - 2203120 2203120
      Anti-Ezrin - 20129 20129
      Anti-Ezrin - 24921 24921
      Anti-Ezrin - 32466 32466
      Anti-Ezrin -2729590 2729590
      Anti-Ezrin -2796487 2796487

      References

      Reference overviewApplicationPub Med ID
      Polycystin-1 binds Par3/aPKC and controls convergent extension during renal tubular morphogenesis.
      Castelli, M; Boca, M; Chiaravalli, M; Ramalingam, H; Rowe, I; Distefano, G; Carroll, T; Boletta, A
      Nature communications  4  2658  2013

      Show Abstract
      Western Blotting, Immunofluorescence24153433 24153433
      Extranuclear signaling of mutated thyroid hormone receptors in promoting metastatic spread in thyroid carcinogenesis.
      Changxue Lu,Sheue-Yann Cheng
      Steroids  76  2011

      Show Abstract
      21473875 21473875
      Proteomics analysis of the ezrin interactome in B cells reveals a novel association with Myo18a?.
      Ken Matsui,Neetha Parameswaran,Nayer Bagheri,Belinda Willard,Neetu Gupta
      Journal of proteome research  10  2011

      Show Abstract
      21751808 21751808
      Beta-catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state.
      Marose, TD; Merkel, CE; McMahon, AP; Carroll, TJ
      Developmental biology  314  112-26  2008

      Show Abstract Full Text Article
      18177851 18177851
      Myc down-regulation as a mechanism to activate the Rb pathway in STAT5A-induced senescence.
      Mallette, FA; Gaumont-Leclerc, MF; Huot, G; Ferbeyre, G
      The Journal of biological chemistry  282  34938-44  2007

      Show Abstract
      17913706 17913706
      Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement
      Potter, D. A., et al
      J Biol Chem, 278:30403-12 (2003)  2003

      Immunofluorescence12764139 12764139
      Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype
      Yang, H. S. and Hinds, P. W.
      Mol Cell, 11:1163-76 (2003)  2003

      Immunoblotting (Western), Immunoprecipitation12769842 12769842
      Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins.
      Tsukita, S and Yonemura, S
      J. Biol. Chem., 274: 34507-10 (1999)  1999

      10574907 10574907

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies