04-1113 | Anti-FADD (NT) Antibody, clone EP887Y, rabbit monoclonal

04-1113
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIH(P), WBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1113
      DescriptionAnti-FADD (NT) Antibody, clone EP887Y, rabbit monoclonal
      Alternate Names
      • FAS-associated death domain protein
      • FAS-associating death domain-containing protein
      • Fas (TNFRSF6)-associated via death domain
      • Fas-associated via death domain
      • Fas-associating protein with death domain
      • Mediator of receptor induced toxicity
      • growth-inhibiting gene 3 protein
      • mediator of receptor-induced toxicity
      Background InformationFADD was originally isolated as a protein that bound to the cytoplasmic domain of Fas in the yeast two-hybrid system. Sequence analysis revealed a region homologous to the death domain of Fas and TNFR-1. Subsequent biochemical studies have shown that FADD associates with FAS through interaction of the death domains. When over expressed in several cell lines, FADD induces apoptosis, which can be blocked by CrmA, an inhibitor of inerleukin-1-beta-converting enzyme. This evidence suggests that FADD plays a role in Fas-mediated apoptosis.
      References
      Product Information
      FormatUnpurified
      Control
      • Jurkat lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-FADD (N-terminal) Antibody, clone EP887Y, Rabbit is validated for use in IH(P), WB for the detection of FADD (N-terminal).
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: 1:250 dilution from a previous lot detected FADD in human lung tissue.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human FADD.
      EpitopeN-terminus
      CloneEP887Y
      HostRabbit
      SpecificityThis antibody recognizes FADD.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GIG3
      • MGC8528
      • MORT1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

      SUBUNIT STRUCTURE: Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.

      TISSUE SPECIFICITY: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

      DOMAIN: Contains a death domain involved in the binding of the corresponding domain within Fas receptor.

      PTM: Phosphorylated.

      SEQUENCE SIMILARITY: Contains 1 death domain.

      Contains 1 DED (death effector) domain.

      Molecular Weight~28 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysates.

      Western Blotting Analysis:
      1:500 dilution of this antibody was used to detect FADD in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications