05-182 | Anti-FAK Antibody, clone 2A7

05-182
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, H, M, RICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-182
      Replaces04-591
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FAK Antibody, clone 2A7
      References
      Product Information
      FormatPurified
      Control
      • Expressed in most tissue, 3T3/A31 cell lysate
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 containing no preservatives.
      Applications
      ApplicationAnti-FAK Antibody, clone 2A7 detects level of FAK & has been published & validated for use in IC & IP.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesNot recommended in Western Blot
      Biological Information
      ImmunogenMixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125FAK.
      Clone2A7
      HostMouse
      SpecificityRecognizes FAK.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
      Gene Symbol
      • PTK2
      • pp125FAK
      • FAK
      • FADK
      • FAK1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      SIZE: 1052 amino acids; 119233 Da
      SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitation of FAK from a mouse 3T3/A31 RIPA cell lysate, subsequently immunobloted with Anti-FAK (Catalog #06-543)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FAK, clone 2A7 (mouse monoclonal IgG1) -2444083 2444083
      Anti-FAK, clone 2A7 - 0701050542 0701050542
      Anti-FAK, clone 2A7 - 16028 16028
      Anti-FAK, clone 2A7 - 16386 16386
      Anti-FAK, clone 2A7 - 17763 17763
      Anti-FAK, clone 2A7 - 19450 19450
      Anti-FAK, clone 2A7 - 2070093 2070093
      Anti-FAK, clone 2A7 - 21121 21121
      Anti-FAK, clone 2A7 - 23166 23166
      Anti-FAK, clone 2A7 - 26136 26136

      References

      Reference overviewApplicationSpeciesPub Med ID
      FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm.
      Bjerke, MA; Dzamba, BJ; Wang, C; DeSimone, DW
      Developmental biology  394  340-56  2014

      Show Abstract
      25127991 25127991
      Ambivalent role of pFAK-Y397 in serous ovarian cancer--a study of the OVCAD consortium.
      Aust, S; Auer, K; Bachmayr-Heyda, A; Denkert, C; Sehouli, J; Braicu, I; Mahner, S; Lambrechts, S; Vergote, I; Grimm, C; Horvat, R; Castillo-Tong, DC; Zeillinger, R; Pils, D
      Molecular cancer  13  67  2014

      Show Abstract
      ImmunohistochemistryHuman24655477 24655477
      Development and characterization of rifampicin-resistant mutants from high virulent strains of Flavobacterium columnare.
      O Olivares-Fuster,C R Arias
      Journal of fish diseases  34  2011

      Show Abstract
      21488906 21488906
      Receptor-interacting protein shuttles between cell death and survival signaling pathways.
      Kamarajan, P; Bunek, J; Lin, Y; Nunez, G; Kapila, YL
      Molecular biology of the cell  21  481-8  2010

      Show Abstract Full Text Article
      19955213 19955213
      Variations of proline-rich kinase Pyk2 expression correlate with prostate cancer progression.
      R Stanzione, A Picascia, P Chieffi, C Imbimbo, A Palmieri, V Mirone, S Staibano, R Franco, G De Rosa, J Schlessinger, D Tramontano
      Laboratory investigation; a journal of technical methods and pathology  81  51-9  2001

      Show Abstract
      11204274 11204274
      E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells
      Kantak, S. S. and Kramer, R. H.
      J Biol Chem, 273:16953-61 (1998)  1998

      Immunoprecipitation9642258 9642258
      Protein-tyrosine phosphatase alpha regulates Src family kinases and alters cell-substratum adhesion
      Harder, K. W., et al
      J Biol Chem, 273:31890-900 (1998)  1998

      Immunoblotting (Western), Immunoprecipitation9822658 9822658
      Insulin receptor substrate-1 as a signaling molecule for focal adhesion kinase pp125(FAK) and pp60(src)
      Lebrun, P., et al
      J Biol Chem, 273:32244-53 (1998)  1998

      Immunoprecipitation9822703 9822703
      Bombesin, vasopressin, lysophosphatidic acid, and sphingosylphosphorylcholine induce focal adhesion kinase activation in intact Swiss 3T3 cells
      Rodriguez-Fernandez, J. L. and Rozengurt, E.
      J Biol Chem, 273:19321-8 (1998)  1998

      Immunoprecipitation9668122 9668122
      Alternatively spliced focal adhesion kinase in rat brain with increased autophosphorylation activity
      Burgaya, F., et al
      J Biol Chem, 272:28720-5 (1997)  1997

      Immunoprecipitation9353341 9353341

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies