05-537 | Anti-FAK Antibody, clone 4.47

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-537
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FAK Antibody, clone 4.47
      Background InformationFocal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.
      Product Information
      • Positive Control Included: 3T3 Cell Lysate (12-305). Other controls: A31 cell lysate, PC-12, Hep-G2, SW 620 and Jurkat RIPA cell lysates
      PresentationProtein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationAnti-FAK Antibody, clone 4.47 is an antibody against FAK for use in IC, IH, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes p125FAK; does not cross react with Pyk-2.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
      Gene Symbol
      • PTK2
      • pp125FAK
      • FAK
      • FADK
      • FAK1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      SIZE: 1052 amino acids; 119233 Da
      SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FAK, clone 4.47 2470904
      Anti-FAK, clone 4.47 - 2153144 2153144
      Anti-FAK, clone 4.47 - 2392294 2392294
      Anti-FAK, clone 4.47 - 2455605 2455605
      Anti-FAK, clone 4.47 - 0608038478 0608038478
      Anti-FAK, clone 4.47 - 0701049384 0701049384
      Anti-FAK, clone 4.47 - 19365 19365
      Anti-FAK, clone 4.47 - 1970370 1970370
      Anti-FAK, clone 4.47 - 2023279 2023279
      Anti-FAK, clone 4.47 - 2037619 2037619


      Reference overviewApplicationSpeciesPub Med ID
      Trop-2 is up-regulated in invasive prostate cancer and displaces FAK from focal contacts.
      Trerotola, M; Ganguly, KK; Fazli, L; Fedele, C; Lu, H; Dutta, A; Liu, Q; De Angelis, T; Riddell, LW; Riobo, NA; Gleave, ME; Zoubeidi, A; Pestell, RG; Altieri, DC; Languino, LR
      Oncotarget  6  14318-28  2015

      Show Abstract
      26015409 26015409
      FAK/PYK2 promotes the Wnt/β-catenin pathway and intestinal tumorigenesis by phosphorylating GSK3β.
      Gao, C; Chen, G; Kuan, SF; Zhang, DH; Schlaepfer, DD; Hu, J
      eLife  4  2015

      Show Abstract
      26274564 26274564
      Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma.
      Stewart, JE; Ma, X; Megison, M; Nabers, H; Cance, WG; Kurenova, EV; Beierle, EA
      Molecular carcinogenesis  54  9-23  2015

      Show Abstract
      23868727 23868727
      Mammary gland-specific ablation of focal adhesion kinase reduces the incidence of p53-mediated mammary tumour formation.
      van Miltenburg, MH; van Nimwegen, MJ; Tijdens, I; Lalai, R; Kuiper, R; Klarenbeek, S; Schouten, PC; de Vries, A; Jonkers, J; van de Water, B
      British journal of cancer  110  2747-55  2014

      Show Abstract
      24809783 24809783
      FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm.
      Bjerke, MA; Dzamba, BJ; Wang, C; DeSimone, DW
      Developmental biology  394  340-56  2014

      Show Abstract
      25127991 25127991
      FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors.
      Megison, ML; Gillory, LA; Stewart, JE; Nabers, HC; Mrozcek-Musulman, E; Beierle, EA
      Molecular cancer research : MCR  12  514-26  2014

      Show Abstract
      24464916 24464916
      Targeted inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines.
      Yoon, H; Choi, YL; Song, JY; Do, I; Kang, SY; Ko, YH; Song, S; Kim, BG
      PloS one  9  e88587  2014

      Show Abstract
      Immunohistochemistry (Paraffin)24523919 24523919
      High focal adhesion kinase expression in breast carcinoma is associated with lymphovascular invasion and triple-negative phenotype.
      Golubovskaya, VM; Ylagan, L; Miller, A; Hughes, M; Wilson, J; Wang, D; Brese, E; Bshara, W; Edge, S; Morrison, C; Cance, WG
      BMC cancer  14  769  2014

      Show Abstract
      ImmunohistochemistryHuman25326692 25326692
      Inhibition of the focal adhesion kinase and vascular endothelial growth factor receptor-3 interaction leads to decreased survival in human neuroblastoma cell lines.
      Beierle, EA; Ma, X; Stewart, JE; Megison, M; Cance, WG; Kurenova, EV
      Molecular carcinogenesis  53  230-42  2014

      Show Abstract
      Western Blotting23065847 23065847
      Proteomic profiling of endothelial invasion revealed receptor for activated C kinase 1 (RACK1) complexed with vimentin to regulate focal adhesion kinase (FAK).
      Dave, JM; Kang, H; Abbey, CA; Maxwell, SA; Bayless, KJ
      The Journal of biological chemistry  288  30720-33  2013

      Show Abstract
      Western Blotting24005669 24005669


      I would like to use this monoclonal antibody on mouse tissue, are there any special procedures I should follow?To reduce the background interaction between the monoclonal antibody and the mouse tissue, you will need to perform antigen retrieval and use a special secondary antibody. For antigen retrieval, prepare a citrate buffer by mixing 19ml of 0.1M citric acid and 82ml of 0.1M sodium citrate to make 1000ml of buffer. Adjust the pH to 6.0 before each use. Add buffer to your slide. Using an 800 watt microwave oven with rotation, microwave for 7.5 min at high, 5 min at 50% (3 cycles), checking the slide at the end of each cycle to see if the solution needs to be replaced, adding water if needed. For the secondary antibody, use a biotinylated isotype specific secondary IGG (IGG-1) and a 3% donkey serum block. This will help reduce the background issues using mouse monoclonals.

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      Alternative Packsize

      Catalogue Number Description  
      05-537-KC Anti-FAK, clone 4.47 (1mg) KC Show Pricing & Availability

      Alternative Format

      Catalogue Number Description  
      16-173 Anti-FAK Antibody, clone 4.47, agarose conjugate Show Pricing & Availability
      16-233 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 488 conjugate Show Pricing & Availability
      16-234 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies