05-118 | Anti-FGF-2/basic FGF Antibody, clone bFM-2

05-118
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, RIHC, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-118
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FGF-2/basic FGF Antibody, clone bFM-2
      OverviewThis antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.
      Alternate Names
      • Basic fibroblast growth factor
      • basic fibroblast growth factor bFGF
      • fibroblast growth factor 2
      • fibroblast growth factor 2 (basic)
      • heparin-binding growth factor 2
      Background InformationFGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      References
      Product Information
      FormatPurified
      Control
      • Fetal liver or kidney tissue.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.
      Applications
      ApplicationDetect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.
      Key Applications
      • Immunohistochemistry
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistochemistry:
      Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
      Biological Information
      ImmunogenPurified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.
      ClonebFM-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects FGF-2/basic FGF.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Rat
      Species Reactivity NoteThis antibody is highly specific for basic FGF from bovine, human, rat, and mouse sources and does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

      Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Gene Symbol
      • FGF2
      • Prostatropin
      • BFGF
      • prostatropin
      • HBGF-2
      • FGFB
      Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
      SIZE: 155 amino acids; 17254 Da
      SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
      SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
      MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
      Molecular Weight17.5 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated on basic FGF (Catalog #01-114).

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FGF-2/basic FGF, clone bFM-2 - 2127773 2127773
      Anti-FGF-2/basic FGF, clone bFM-2 - 2387480 2387480
      Anti-FGF-2/basic FGF, clone bFM-2 - 2453128 2453128
      Anti-FGF-2/basic FGF, clone bFM-2 - DAM1749914 DAM1749914
      Anti-FGF-2/basic FGF, clone bFM-2 - 15291 15291
      Anti-FGF-2/basic FGF, clone bFM-2 - 16397 16397
      Anti-FGF-2/basic FGF, clone bFM-2 - 18292 18292
      Anti-FGF-2/basic FGF, clone bFM-2 - 18957 18957
      Anti-FGF-2/basic FGF, clone bFM-2 - 19916 19916
      Anti-FGF-2/basic FGF, clone bFM-2 - 2003655 2003655

      References

      Reference overviewApplicationPub Med ID
      High molecular weight fibroblast growth factor-2 in the human heart is a potential target for prevention of cardiac remodeling.
      Santiago, JJ; McNaughton, LJ; Koleini, N; Ma, X; Bestvater, B; Nickel, BE; Fandrich, RR; Wigle, JT; Freed, DH; Arora, RC; Kardami, E
      PloS one  9  e97281  2014

      Show Abstract
      Western Blotting24827991 24827991
      EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1.
      Sáinz-Jaspeado, M; Huertas-Martinez, J; Lagares-Tena, L; Martin Liberal, J; Mateo-Lozano, S; de Alava, E; de Torres, C; Mora, J; Del Muro, XG; Tirado, OM
      PloS one  8  e71449  2013

      Show Abstract
      23951165 23951165
      Increased fibroblast telomerase expression precedes myofibroblast α-smooth muscle actin expression in idiopathic pulmonary fibrosis.
      Waisberg, DR; Parra, ER; Barbas-Filho, JV; Fernezlian, S; Capelozzi, VL
      Clinics (São Paulo, Brazil)  67  1039-46  2012

      Show Abstract
      23018301 23018301
      Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.
      Mai-Khoi Q DO,Takahiro Suzuki,Borjigin Gerelt,Yusuke Sato,Wataru Mizunoya,Mako Nakamura,Yoshihide Ikeuchi,Judy E Anderson,Ryuichi Tatsumi
      Animal science journal = Nihon chikusan Gakkaihō  83  2012

      Show Abstract
      23035711 23035711
      Differential gene expression in eyecup and retina of a mouse model of Stargardt-like macular dystrophy (STGD3).
      Kuny, S; Gaillard, F; Sauvé, Y
      Investigative ophthalmology & visual science  53  664-75  2012

      Show Abstract
      22199241 22199241
      Preferential accumulation and export of high molecular weight FGF-2 by rat cardiac non-myocytes.
      Santiago, JJ; Ma, X; McNaughton, LJ; Nickel, BE; Bestvater, BP; Yu, L; Fandrich, RR; Netticadan, T; Kardami, E
      Cardiovascular research  89  139-47  2011

      Show Abstract
      Western Blotting20696821 20696821
      Renal collecting system growth and function depend upon embryonic γ1 laminin expression.
      Yang, DH; McKee, KK; Chen, ZL; Mernaugh, G; Strickland, S; Zent, R; Yurchenco, PD
      Development (Cambridge, England)  138  4535-44  2011

      Show Abstract
      21903675 21903675
      Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells.
      Candela, Maria Elena, et al.
      J. Cell. Physiol., 224: 144-51 (2010)  2010

      Show Abstract
      20232295 20232295
      Matrix metalloproteinases and angiogenic factors: predictors of survival after radical prostatectomy for clinically organ-confined prostate cancer?
      Boxler, S; Djonov, V; Kessler, TM; Hlushchuk, R; Bachmann, LM; Held, U; Markwalder, R; Thalmann, GN
      The American journal of pathology  177  2216-24  2010

      Show Abstract
      Immunohistochemistry20889560 20889560
      beta1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity.
      Xi Zhang,Glenda Mernaugh,Dong-Hua Yang,Leslie Gewin,Manakan B Srichai,Raymond C Harris,Juan M Iturregui,Raoul D Nelson,Donald E Kohan,Dale Abrahamson,Reinhard Fässler,Peter Yurchenco,Ambra Pozzi,Roy Zent
      Development (Cambridge, England)  136  2009

      Show Abstract Full Text Article
      19710172 19710172

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies