05-1466 | Anti-FOXA1 Antibody, clone 2F83

05-1466
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1466
      DescriptionAnti-FOXA1 Antibody, clone 2F83
      Alternate Names
      • Forkhead box protein A1
      • forkhead box A1
      • hepatocyte nuclear factor 3, alpha
      Background InformationFOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER with chromatin (Badve, 2007).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemisty:
      Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized.
      Biological Information
      ImmunogenRecombinant human GST-FOXA1 protein encompassing amino acids 7-86.
      EpitopeN-terminus
      Clone2F83
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects human FOXA1 (HNF3α).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and Mouse. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]
      Gene Symbol
      • HNF-3-alpha
      • HNF-3A
      • HNF3A
      • TCF3A
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes.

      SUBCELLULAR LOCATION: Nucleus.

      SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.

      Molecular Weight49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HeLa nuclear extracts.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FOXA1, clone 2F83 2465169
      Anti-FOXA1, clone 2F83 - 2410226 2410226
      Anti-FOXA1, clone 2F83 - 2460942 2460942
      Anti-FOXA1, clone 2F83 - 2038291 2038291
      Anti-FOXA1, clone 2F83 - NG1605288 NG1605288
      Anti-FOXA1, clone 2F83 - NG1752513 NG1752513
      Anti-FOXA1, clone 2F83 -2499582 2499582
      Anti-FOXA1, clone 2F83 -2529401 2529401
      Anti-FOXA1, clone 2F83 -2533835 2533835
      Anti-FOXA1, clone 2F83 -2535374 2535374

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      05-1466-KC Anti-FOXA1, clone 2F83 (KC) Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies