04-960 | Anti-FOXP3 Antibody, clone 3G3

04-960
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HFC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-960
      DescriptionAnti-FOXP3 Antibody, clone 3G3
      Alternate Names
      • forkhead box P3
      • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
      • immunodeficiency, polyendocrinopathy, enteropathy, X-linked
      Background InformationThe regulation of tolerance to self-antigens is a necessary biological process in order to prevent autoimmunity. CD4+ CD25+ regulatory T cells are known regulators of immune tolerance and activation. In both human and mice species, CD4+ CD25+ regulatory T cells are consistent in their expression of the transcription factor FOXP3 (forkhead box P3). FOXP3 acts as the “master switch” for the function and development of regulatory T cells. Mutations and deletions in the sequence encoding for FOXP3 expression lead to an inability to express FOXP3, causing an inability to regulate T cell development and leading to fatal autoimmune diseases such as IPEX and XLAAD. Conversely, recent studies have shown high and wide expression of FOXP3 in several cancer cell lines and tumors, indicating that FOXP3 is involved in preventing immunity against malignant tumors.
      References
      Product Information
      FormatPurified
      Control
      • Mouse CD4+ CD25+ splenocytes
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-FOXP3 Antibody, clone 3G3 is a Mouse Monoclonal Antibody for detection of FOXP3 also known as forkhead box P3 & has been validated in FC, ELISA.
      Key Applications
      • Flow Cytometry
      • ELISA
      Application NotesFlow Cytometry Analysis: 1 µg from a previous lot of a biotinylated version of this antibody detected FOXP3 in human PBMC's. THIS ANTIBODY IS NOT BIOTINYLATED.

      ELISA: A previous lot of this antibody was shown to work in ELISA by an independent laboratory (Gavin, M., et al., 2006).
      Biological Information
      ImmunogenHistadine-tagged recombinant protein corresponding to the N-terminus of mouse FOXP3.
      EpitopeN-terminus
      Cloneclone 3G3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of Forkhead box protein P3 (FOXP3).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • FOXP3
      • AIID
      • DIETER
      • IPEX
      • JM2
      • PIDX
      • SCURFIN
      • Scurfin
      • XPID
      • scurfin
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Probable transcription factor. Plays a critical role in the control of immune response.

      SUBCELLULAR LOCATION: Nucleus Potential.

      TISSUE SPECIFICITY: High level of expression in thymus and spleen.

      INVOLVEMENT IN DISEASE: Defects in Foxp3 are the cause of the scurfy phenotype (sf). It results in a lethal disorder of immunoregulation, characterized by infections, diarrhea, anemia, thrombocytopenia, hypogonadism, gastrointestinal bleeding, lymphadenopathy and leukocytosis.

      SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger.

      Contains 1 fork-head DNA-binding domain.
      Molecular WeightExpected molecular weight ~47 kDa.
      On western blot, a molecular weight of ~49-55 kDa may be observed (Bolzer, K., et al., 2009).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse CD4+ CD25+ splenocytes.

      Flow Cytometry Analysis: 5 µg of a biotinylated version of this antibody detected FOXP3 in mouse CD4+ CD25+ splenocytes.
      THIS ANTIBODY IS NOT BIOTINYLATED.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies