05-338 | Anti-Fas Antibody (human, neutralizing), clone ZB4

05-338
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-338
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Fas Antibody (human, neutralizing), clone ZB4
      OverviewThis product does not induce apoptosis in cell culture.
      Alternate Names
      • APO-1 cell surface antigen
      • Apo-1 antigen
      • Apoptosis-mediating surface antigen FAS
      • CD95 antigen
      • FASLG receptor
      • Fas (TNF receptor superfamily, member 6)
      • Fas AMA
      • Fas antigen
      • apoptosis antigen 1
      • tumor necrosis factor receptor superfamily member 6
      • tumor necrosis factor receptor superfamily, member 6
      Background InformationFas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.
      References
      Product Information
      FormatPurified
      Control
      • Various human cells
      PresentationAmmonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.
      Applications
      ApplicationAnti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Neutralizing
      Application NotesFlow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.
      Biological Information
      ImmunogenRecombinant human Fas.
      Cloneclone ZB4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. This antibody does not recognize TNF and mouse Fas.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
      Gene Symbol
      • FAS
      • OTTHUMP00000059646
      • LFG
      • FASTM
      • ALPS1A
      • TNFRSF6
      • CD95
      • FAS1
      • FAIM2
      • APT1
      • NMP35
      • APO-1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
      SIZE: 335 amino acids; 37732 Da
      SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
      TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
      DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
      DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
      SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
      Molecular Weight45 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Quality AssuranceEvaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

      Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Fas (human, neutralizing), clone ZB4 - 2120009 2120009
      Anti-Fas (human, neutralizing), clone ZB4 - 2135062 2135062
      Anti-Fas (human, neutralizing), clone ZB4 - 2139985 2139985
      Anti-Fas (human, neutralizing), clone ZB4 - 2392215 2392215
      Anti-Fas (human, neutralizing), -2554838 2554838
      Anti-Fas (human, neutralizing), -2577037 2577037
      Anti-Fas (human, neutralizing), -2585868 2585868
      Anti-Fas (human, neutralizing), -2602776 2602776
      Anti-Fas (human, neutralizing), -2659141 2659141
      Anti-Fas (human, neutralizing), -2659698 2659698

      References

      Reference overviewApplicationPub Med ID
      Networked T cell death following macrophage infection by Mycobacterium tuberculosis.
      Macdonald, SH; Woodward, E; Coleman, MM; Dorris, ER; Nadarajan, P; Chew, WM; McLaughlin, AM; Keane, J
      PloS one  7  e38488  2012

      Show Abstract
      22675566 22675566
      p53-mediated delayed NF-κB activity enhances etoposide-induced cell death in medulloblastoma.
      Meley, D; Spiller, DG; White, MR; McDowell, H; Pizer, B; Sée, V
      Cell death & disease  1  e41  2010

      Show Abstract
      21364648 21364648
      Elucidation of molecular events leading to neutrophil apoptosis following phagocytosis: cross-talk between caspase 8, reactive oxygen species, and MAPK/ERK activation.
      Zhang, Bin, et al.
      J. Biol. Chem., 278: 28443-54 (2003)  2003

      Show Abstract
      12736263 12736263
      Molecular determinants of apoptosis induced by the cytotoxic ribonuclease onconase: evidence for cytotoxic mechanisms different from inhibition of protein synthesis.
      M S Iordanov, O P Ryabinina, J Wong, T H Dinh, D L Newton, S M Rybak, B E Magun
      Cancer research  60  1983-94  2000

      Show Abstract
      10766189 10766189
      Regulation of Fas ligand expression and cell death by apoptosis-linked gene 4
      Lacana, E. and D'Adamio, L.
      Nat Med, 5:542-7 (1999)  1999

      Neutralization Assay10229231 10229231
      Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis
      Boirivant, M., et al
      J Clin Invest, 98:2616-22 (1996)  1996

      Neutralization Assay8958226 8958226
      Involvement of apoptosis antigen Fas in clonal deletion of human thymocytes
      Yonehara, S, et al
      Int Immunol, 6:1849-56 (1994)  1994

      7535095 7535095

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-351 Anti-Fas Antibody, clone 7C10 Show Pricing & Availability
      AB16982 Anti-Fas Ligand Antibody Show Pricing & Availability
      FCMAB212P Milli-Mark™ Anti-CD95-PE Antibody, clone DX2 Show Pricing & Availability
      MAB3061 Anti-Fas Antibody, clone SM1/1 Show Pricing & Availability
      MABC763 Anti-CD95, clone EPR5700, Rabbit Monoclonal Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies