04-592 | Anti-Flotillin-1 Antibody, rabbit monoclonal, clone EP446Y

04-592
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-592
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Flotillin-1 Antibody, rabbit monoclonal, clone EP446Y
      Alternate Names
      • Reggie-2
      Background InformationFlotillin-1, also known as Reggie-2, belongs to a larger family of proteins that share an evolutionarily conserved stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (1). Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. Flotillin was first found in cells that lack morphologically definable caveolae, as well as in non-caveolar membranes (2) and was subsequently identified in low density detergent-insoluble complexes of endothelial cells, where they were termed flotillins (3). As well as its postulated role in neuronal regeneration, flotilllin-1 has been implicated in insulin signaling to trigger glucose transporter redistribution in adipocytes (4) Flotillin also associates with phagosomes in macrophages (5).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Flotillin-1 Antibody, Rabbit, clone EP446Y is validated for use in WB for the detection of Flotillin-1.
      Key Applications
      • Western Blotting
      Application NotesWB: 1:500
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues in human Flotillin-1.
      CloneEP446Y
      HostRabbit
      SpecificityRecognizes Human, Mouse Flotillin-1.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse (positive control: K562 cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCaveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.
      Gene Symbol
      • FLOT1
      • Flotillin-1.
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O75955 # May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
      SIZE: 427 amino acids; 47355 Da
      SUBUNIT: Hetero-oligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane, caveola; Peripheral membrane protein. Melanosome. Note=Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: O75955 ## Belongs to the band 7/mec-2 family. Flotillin subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Flotillin-1, clone EP446Y 2479774
      Anti-Flotillin-1, clone EP446Y (Rabbit monoclonal) - 2139979 2139979
      Anti-Flotillin-1, clone EP446Y (Rabbit monoclonal) - 2343877 2343877
      Anti-Flotillin-1, clone EP446Y (Rabbit monoclonal) - 2208755 2208755
      Anti-Flotillin-1, clone EP446Y (Rabbit monoclonal) - DAM1411359 DAM1411359
      Anti-Flotillin-1, clone EP446Y - DAM1467589 DAM1467589
      Anti-Flotillin-1, clone EP446Y - DAM1778053 DAM1778053

      References

      Reference overviewPub Med ID
      Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes
      Dermine, J F, et al
      J Biol Chem, 276:18507-12 (2001)  2001

      11279173 11279173
      Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2.
      Stuermer, C A, et al.
      Mol. Biol. Cell, 12: 3031-45 (2001)  2001

      Show Abstract
      11598189 11598189
      CAP defines a second signalling pathway required for insulin-stimulated glucose transport
      Baumann, C A, et al
      Nature, 407:202-7 (2000)  2000

      11001060 11001060
      The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins
      Tavernarakis, N, et al
      Trends Biochem Sci, 24:425-7 (1999)  1999

      10542406 10542406
      Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins
      Bickel, P E, et al
      J Biol Chem, 272:13793-802 (1997)  1997

      9153235 9153235

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies