MABE985 | Anti-Fox1 Antibody, clone 1D10

MABE985
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IHC, ICC, RIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE985
      DescriptionAnti-Fox1 Antibody, clone 1D10
      Alternate Names
      • RNA binding protein fox-1 homolog 1
      • Ataxin-2-binding protein 1
      • Fox-1 homolog A
      • Fox1
      Background InformationRNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • RNA Binding Protein Immunoprecipitation (RIP)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Fox1 in 10 µg of adult mouse frontal cortex tissue lysate.
      Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
      Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293).
      RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711).
      Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse Fox1.
      Clone1D10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Rbfox1
      • A2bp
      • A2bp1
      • Fox1
      • MNCb-3035
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in adult mouse brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies