04-1031 | Anti-GFAP (CT) Antibody, clone EP672Y, rabbit monoclonal

04-1031
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P), ICC, IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1031
      DescriptionAnti-GFAP (CT) Antibody, clone EP672Y, rabbit monoclonal
      Alternate Names
      • glial fibrillary acidic protein
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      References
      Product Information
      FormatUnpurified
      Control
      • Rat brain lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-GFAP (C-term) Antibody, clone EP672Y, Rabbit is validated for use in WB, IH(P), IC, IP for the detection of GFAP (C-term).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry: A 1:250-500 dilution of a previous lot was used in IC.
      Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.
      Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected GFAP in human brain tissue.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues in the C-terminus of human GFAP.
      EpitopeC-terminus
      CloneEP672Y
      HostRabbit
      SpecificityThis antibody recognizes GFAP at and around the c-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
      Gene Symbol
      • FLJ45472
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SUBUNIT STRUCTURE: Interacts with SYNM By similarity. Isoform 3 interacts with PSEN1 (via N-terminus).
      SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with intermediate filaments.
      TISSUE SPECIFICITY: Expressed in cells lacking fibronectin.
      INVOLVEMENT IN DISEASE: Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.
      Molecular Weight~50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Rat brain cell lysates.
      Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect GFAP in rat brain cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GFAP (C-term), clone EP672Y - NG1820808 NG1820808
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclona 2464507
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2395661 2395661
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2420375 2420375
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2424631 2424631
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2441877 2441877
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2453104 2453104
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 1953024 1953024
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2110541 2110541
      Anti-GFAP (C-term), clone EP672Y, Rabbit Monoclonal - 2199725 2199725

      References

      Reference overviewPub Med ID
      The problem of axonal injury in the brains of veterans with histories of blast exposure.
      Ryu, J; Horkayne-Szakaly, I; Xu, L; Pletnikova, O; Leri, F; Eberhart, C; Troncoso, JC; Koliatsos, VE
      Acta neuropathologica communications  2  153  2014

      Show Abstract
      25422066 25422066

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies