MABS508 | Anti-GOT1 Antibody, clone 1F5.2

MABS508
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS508
      DescriptionAnti-GOT1 Antibody, clone 1F5.2
      Alternate Names
      • Cysteine aminotransferase, cytoplasmic
      • Cysteine transaminase, cytoplasmic
      • cCAT
      • Glutamate oxaloacetate transaminase 1
      • Transaminase A
      • Aspartate aminotransferase, cytoplasmic
      • cAspAT
      • GOT1
      Background InformationAspartate aminotransferase, cytoplasmic (EC 2.6.2.1.; EC 2.6.2.3; UniProt P17174; also known as Aspartate aminotransferase 1, Aspartate transaminase 1, cAspAT, cCAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase cytoplasmic, Glutamate oxaloacetate transaminase 1, Growth-inhibiting protein 18, Transaminase A) is encoded by the GOT1 (also known as GIG18, ASTQTL1) gene (Gene ID 2805) in human. Aspartate aminotransferase is expressed in many types of cells and tissues and is conserved among prokaryotic and eukaryotic organisms. There exist two highly similar AST forms in mammals, the cytosolic cAST (encoded by the GOT1 gene) and the mitochondrial mAST (encoded by the GOT2 gene). Serum aspartate aminotransferase activity is largely that of cAST/GOT1, and elevated serum AST activity is associated with liver, heart, and muscle injury. AST catalyzes the reversible conversion between aspartate/alpha-ketoglutarate and oxaloacetate/glutamate, increasing oxaloacetate concentration therefore shifts the equilibrium of AST-catalzed reaction toward aspartate/alpha-ketoglutarate and away from glutatmate. Lowering blood glutamate concentration via oxaloacetate i.v. injection prompts an efflux of excess extracellular brain glutamate into the blood, which is reported to allevate the infarct size and edema in a rat model of ischemia induced by transient occlusion of the middle cerebral artery occlusion (MCAO).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect GOT1 using this mouse monoclonal Anti-GOT1 Antibody, clone 1F5.2, Cat. No. MABS508, validated for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GOT1 in human liver hepatocytes and in human cerebellum.
      Biological Information
      ImmunogenGST-tagged Recombinant protein corresponding to the N-terminal region of human GOT1.
      EpitopeN-terminal half
      Clone1F5.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GOT1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected GOT1 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications