05-465 | Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

05-465
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Ch, Po, RbIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-465
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1
      Alternate Names
      • G-protein coupled receptor kinase 2, adrenergic, beta
      • receptor kinase 1
      • beta adrenergic receptor kinase 1
      Background InformationThe beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
      GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors.
      References
      Product Information
      FormatPurified
      Control
      • Human breast carcinoma tissue, mouse heart tissue, SK OV 3 cell lysates and NIH 3T3 cell lysates.
        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect GRK 2/3 (βARK 1/2) using this Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A previous lot of this antibody was used to immunoprecipitate GRK2/3 from transfected cells, as shown by an independent laboratory.
      Biological Information
      ImmunogenPartial GST fusion protein corresponding to the GRK3 (βARK2) carboxyl terminus (a.a. 467-688). This immunogen shares homology with GRK2 (βARK1). The minimal epitope recognized by the antibody is a.a. 483-485. Clone C5/1.1.
      Epitopea.a. 483-485
      CloneC5/1.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes GRK2/3, Mr 80 kDa. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Chicken
      • Pig
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
      Gene Symbol
      • ADRBK1
      • Beta-ARK-1
      • GRK2
      • BARK
      • FLJ16718
      • BETA-ARK1
      • BARK1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25098 # Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.
      SIZE: 689 amino acids; 79574 Da
      SUBUNIT: Interacts with GIT1 (By similarity).
      SIMILARITY: SwissProt: P25098 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain. ... hide » see more »
      Molecular Weight80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts, bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma or human HeLa cell lysates.

      Western Blot Analysis:
      0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GRK 2/3 (#946;ARK 1/2), clone C5/1.1 - DAM1805225 DAM1805225
      Anti-GRK 2/3 (ARK 1/2), clone C5/1.1 - DAM1612179 DAM1612179
      Anti-GRK 2/3 (ARK 1/2), clone C5/1.1 - DAM1718039 DAM1718039
      Anti-GRK 2/3 (ARK 1/2), clone C5/1.1 - JBC1395510 JBC1395510
      Anti-GRK 2/3 (ARK 1/2), clone C5/1.1 - JBC1888204 JBC1888204
      Anti-GRK 2/3 (ARK 1/2), clone C5/1.1 - JBC1940042 JBC1940042
      Anti-GRK 2/3 (bARK 1/2), clone C5/1.1 (mouse monoclonal IgG2ak) - JBC1395510 JBC1395510
      Anti-GRK 2/3 (beta;ARK 1/2), clone C5/1.1 - 16392 16392
      Anti-GRK 2/3 (beta;ARK 1/2), clone C5/1.1 - 18695 18695
      Anti-GRK 2/3 (beta;ARK 1/2), clone C5/1.1 - 19855 19855

      References

      Reference overviewApplicationSpeciesPub Med ID
      Desensitization and internalization of endothelin receptor A: impact of G protein-coupled receptor kinase 2 (GRK2)-mediated phosphorylation.
      Gärtner, F; Seidel, T; Schulz, U; Gummert, J; Milting, H
      The Journal of biological chemistry  288  32138-48  2013

      Show Abstract
      Western Blotting24064210 24064210
      Toll-like receptors differentially regulate GPCR kinases and arrestins in primary macrophages.
      Loniewski, Katie, et al.
      Mol. Immunol., 45: 2312-22 (2008)  2008

      Show Abstract
      Mouse18180038 18180038
      Maternal low-protein diet programs cardiac beta-adrenergic response and signaling in 3-mo-old male offspring.
      Fernandez-Twinn, DS; Ekizoglou, S; Wayman, A; Petry, CJ; Ozanne, SE
      American journal of physiology. Regulatory, integrative and comparative physiology  291  R429-36  2006

      Show Abstract
      16914429 16914429
      Phosphorylation and desensitization of human endothelin A and B receptors. Evidence for G protein-coupled receptor kinase specificity.
      Freedman, N J, et al.
      J. Biol. Chem., 272: 17734-43 (1997)  1997

      Show Abstract
      9211925 9211925
      Receptor and G betagamma isoform-specific interactions with G protein-coupled receptor kinases.
      Daaka, Y, et al.
      Proc. Natl. Acad. Sci. U.S.A., 94: 2180-5 (1997)  1997

      Show Abstract
      9122168 9122168
      Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases.
      Oppermann, M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 93: 7649-54 (1996)  1996

      Show Abstract
      8755530 8755530
      Inhibition of G protein-coupled receptor kinase subtypes by Ca2+/calmodulin.
      Chuang, T T, et al.
      J. Biol. Chem., 271: 28691-6 (1996)  1996

      8910504 8910504
      G protein-coupled receptor kinase GRK2 is a phospholipid-dependent enzyme that can be conditionally activated by G protein betagamma subunits.
      DebBurman, S K, et al.
      J. Biol. Chem., 271: 22552-62 (1996)  1996

      Show Abstract
      8798423 8798423

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MABS155 Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies