06-332 | Anti-GST Tag Antibody

06-332
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-332
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GST Tag Antibody
      References
      Product Information
      FormatPurified
      Control
      • Cells expressing the GST-tag
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-GST Tag Antibody detects level of GST Tag & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST (Glutathione-S-transferase) derived from the pGEX expression vector and linked to KLH
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes GST-fusion proteins.
      IsotypeIgG
      Species Reactivity
      • All
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
      Gene Symbol
      • GSTP1
      • DFN7
      • GST3
      • FAEES3
      • GSTP1-1
      • PI
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09211 # Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
      SIZE: 210 amino acids; 23356 Da
      SUBUNIT: Homodimer.
      SIMILARITY: SwissProt: P09211 ## Belongs to the GST superfamily. Pi family.
      Molecular Weight28-30 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on MAP Kinase 2/Erk2, active (Catalog # 14-173). 0.5-2mg/ml of a previous lot detected 500ng of GST (Catalog # 12-350)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GST 2470862
      Anti-GST (rabbit polyclonal IgG) 2943743
      Anti-GST (rabbit polyclonal IgG) 2885513
      Anti-GST (rabbit polyclonal IgG) 2927683
      Anti-GST - DAM1567247 DAM1567247
      Anti-GST Tag - 16389 16389
      Anti-GST Tag - 18084 18084
      Anti-GST Tag - 20969 20969
      Anti-GST Tag - 22583 22583
      Anti-GST Tag - 23321 23321

      References

      Reference overviewApplicationSpeciesPub Med ID
      Intracellular Analysis of the Interaction between the Human Papillomavirus Type 16 E6 Oncoprotein and Inhibitory Peptides.
      Stutz, C; Reinz, E; Honegger, A; Bulkescher, J; Schweizer, J; Zanier, K; Travé, G; Lohrey, C; Hoppe-Seyler, K; Hoppe-Seyler, F
      PloS one  10  e0132339  2015

      Show Abstract
      26151636 26151636
      Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.
      Jin, H; Chiou, TT; Serwanski, DR; Miralles, CP; Pinal, N; De Blas, AL
      The Journal of biological chemistry  289  29420-36  2014

      Show Abstract
      25193658 25193658
      Novel somatic single nucleotide variants within the RNA binding protein hnRNP A1 in multiple sclerosis patients.
      Lee, S; Levin, M
      F1000Research  3  132  2014

      Show Abstract
      Immunofluorescence25254102 25254102
      Tyrosine phosphorylation of the orphan receptor ESDN/DCBLD2 serves as a scaffold for the signaling adaptor CrkL.
      Aten, TM; Redmond, MM; Weaver, SO; Love, CC; Joy, RM; Lapp, AS; Rivera, OD; Hinkle, KL; Ballif, BA
      FEBS letters  587  2313-8  2013

      Show Abstract
      23770091 23770091
      Lotus japonicus E3 ligase SEVEN IN ABSENTIA4 destabilizes the symbiosis receptor-like kinase SYMRK and negatively regulates rhizobial infection.
      Den Herder, G; Yoshida, S; Antolín-Llovera, M; Ried, MK; Parniske, M
      The Plant cell  24  1691-707  2012

      Show Abstract
      22534128 22534128
      Revisiting G3BP1 as a RasGAP binding protein: sensitization of tumor cells to chemotherapy by the RasGAP 317-326 sequence does not involve G3BP1.
      Annibaldi, A; Dousse, A; Martin, S; Tazi, J; Widmann, C
      PloS one  6  e29024  2011

      Show Abstract
      22205990 22205990
      Regulation of nuclear import/export of carbohydrate response element-binding protein (ChREBP): interaction of an alpha-helix of ChREBP with the 14-3-3 proteins and regulation by phosphorylation.
      Sakiyama, H; Wynn, RM; Lee, WR; Fukasawa, M; Mizuguchi, H; Gardner, KH; Repa, JJ; Uyeda, K
      The Journal of biological chemistry  283  24899-908  2008

      Show Abstract
      Western Blotting18606808 18606808
      Proteomic analysis of bovine sperm YWHA binding partners identify proteins involved in signaling and metabolism.
      Puri, P; Myers, K; Kline, D; Vijayaraghavan, S
      Biology of reproduction  79  1183-91  2008

      Show Abstract
      Western BlottingBovine18753613 18753613
      The telomeric protein TRF2 binds the ATM kinase and can inhibit the ATM-dependent DNA damage response.
      Karlseder, J; Hoke, K; Mirzoeva, OK; Bakkenist, C; Kastan, MB; Petrini, JH; de Lange, T
      PLoS biology  2  E240  2004

      Show Abstract
      Western Blotting15314656 15314656
      Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland.
      Xinbo Li, Carl Olson, Shijun Lu, James I Nagy
      Histochemistry and cell biology  122  485-98  2004

      Show Abstract
      15558297 15558297

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies