05-1534 | Anti-Gemin4 Antibody, clone 17D10

05-1534
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1534
      DescriptionAnti-Gemin4 Antibody, clone 17D10
      Alternate Names
      • HCC-associated protein 1
      • component of gems 4
      • gem (nuclear organelle) associated protein 4
      • gemin 4
      Background InformationGemin4, also known as Component of Gems 4, is a component of the SMN complex. It is found in the cytoplasm, nucleoli, and to nuclear bodies called Gemini bodies (gems). As part of a complex this protein functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. Gemin4 directly interacts with the DEAD box protein, Gemin3, as well as other snRNP proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE.
      The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence found in small nuclear RNAs. Reduced levels of SMN cause the motor neuron degenerative disease, spinal muscular atrophy.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Gemin4 Antibody, clone 17D10 (mouse monoclonal antibody) validated in WB, IP to detect Gemin4 also known as HCC-associated protein 1, component of gems 4, gem (nuclear organelle) associated protein 4.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      Reported by external laboratory using representative lot of this antibody.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human Gemin4.
      EpitopeUnknown
      Clone17D10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Gemin4.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
      Gene Symbol
      • GEMIN4
      • Gemin-4
      • HC56
      • HCAP1
      • HHRF-1
      • p97
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.

      SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN3 and with several snRNP SM core proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE. Interacts with PPP4R2.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus › nucleolus. Nucleus › gem. Note: Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus.
      Molecular Weight~ 120 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1 µg/ml of this antibody detected Gemin4 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications