05-1535 | Anti-Gemin5 Antibody, clone 10G11

05-1535
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1535
      DescriptionAnti-Gemin5 Antibody, clone 10G11
      Alternate Names
      • gem (nuclear organelle) associated protein 5
      Background InformationGemin5 is the snRNA-binding component of the SMN complex. The survival of motor neurons (SMN) complex is essential for the biogenesis of small nuclear ribonucleoprotein (snRNP) complexes in eukaryotic cells. Reduced levels of SMN cause the motor neuron degenerative disease, spinal muscular atrophy. As part of this complex, Gemin5 is found in the cytoplasm, nucleoli, and in nuclear bodies called Gemini bodies (gems). Gemin5-containing subunits bind small nuclear RNA independently of the SMN complex and without a requirement for exogenous ATP. ATP hydrolysis is, however, required for displacement of small nuclear RNAs from the Gemin5-containing subunits and their assembly into snRNPs.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified Mouse Monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Gemin5 Antibody, clone 10G11 is a Mouse Monoclonal Antibody for detection of Gemin5 also known as gem (nuclear organelle) associated protein 5 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry:
      A representative lot of this antibody was used by an independent laboratory for immunocytochemistry analysis. See Feng, W., et al. (2005). Hum. Mol. Genet. 14: 1605 - 1611.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human Gemin5.
      EpitopeUnknown
      Clone10G11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Gemin5.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM].
      Gene Symbol
      • gemin 5
      • gemin5
      • GEMIN5
      • MGC142174
      • DKFZp586M1824
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.

      SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with SMN1, SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE.

      SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Nucleus › gem. Cytoplasm. Note: Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.

      SEQUENCE SIMILARITIES: Belongs to the WD repeat gemin-5 family.

      Contains 13 WD repeats.
      Molecular Weight~ 169 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Gemin5 on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Gemin5, clone 10G11 - 2427468 2427468
      Anti-Gemin5, clone 10G11 - 2021740 2021740
      Anti-Gemin5, clone 10G11 - 2201498 2201498
      Anti-Gemin5, clone 10G11 - 2282186 2282186
      Anti-Gemin5, clone 10G11 - 2295481 2295481
      Anti-Gemin5, clone 10G11 - 2320075 2320075
      Anti-Gemin5, clone 10G11 - 2495670 2495670
      Anti-Gemin5, clone 10G11 - NRG1721910 NRG1721910
      Anti-Gemin5, clone 10G11 -2594343 2594343
      Anti-Gemin5, clone 10G11 Monoclonal Antibody 2964472