05-1403 | Anti-Giα2 Antibody, clone L5

05-1403
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-14013 is a recommended replacement for MAB3077

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, B, M, H, Gp, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1403
      ReplacesMAB3077
      DescriptionAnti-Giα2 Antibody, clone L5
      References
      Product Information
      FormatPurified
      Control
      • Huvec cell lysate
      PresentationPurified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesOptimal working dilutions must be determined by end user.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.
      Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

      Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
      Biological Information
      ImmunogenRecombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
      CloneL5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Giα-2.
      IsotypeIgG2b
      Species Reactivity
      • Rat
      • Bovine
      • Mouse
      • Human
      • Guinea Pig
      • Rat
      Species Reactivity NoteStrong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GNAI2
      • GNAI2B
      • WUGSC:H_LUCA15.1
      • H_LUCA15.1
      • H_LUCA16.1
      • GIP
      • WUGSC:H_LUCA16.1
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04899 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
      SIZE: 355 amino acids; 40451 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with UNC5B.
      SIMILARITY: SwissProt: P04899 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Molecular WeightLabels a single protein band of 39-42 kDa in bovine and rat membranes.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Huvec lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      MAB3077 Anti-G Protein Giα-2 Antibody, clone L5 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies