CBL154P | Anti-H-CAM Antibody, clone F10-44-2, Phycoerythrin conjugated

CBL154P
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberCBL154P
      ReplacesMAB2135E
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-H-CAM Antibody, clone F10-44-2, Phycoerythrin conjugated
      Alternate Names
      • CD44
      References
      Product Information
      FormatPhycoerythrin
      PresentationThe conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      ApplicationAnti-H-CAM Antibody, clone F10-44-2, Phycoerythrin conjugated detects level of H-CAM & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Application NotesIdentification of CD44 positive cells by flow cytometry and immunocytochemistry

      Studies involving T, B cell, monocyte and granulocyte binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites

      Studies on HCAM (homing receptor) function

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified human T cells
      CloneF10-44-2
      HostMouse
      SpecificityThe CD44 antigen is a transmembranous glycoprotein of 80 kDa, which has undergone both extensive O-linked and N-linked glycosylation. This antibody is specific for epitope 1. The CD44 antigen is present on T lymphocytes, granulocytes, red blood cells, brain and epithelial cells. There is weak expression on platelets. FUSION PARTNER: NS1 myeloma cell line
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
      Gene Symbol
      • CD44
      • LHR
      • HUTCH-I
      • CDW44
      • CDw44
      • ECMR-III
      • Epican
      • MGC10468
      • CD44R
      • MDU2
      • PGP-1
      • CSPG8
      • Pgp1
      • IN
      • MUTCH-I
      • MDU3
      • MIC4
      • MC56
      • HCELL
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
      SIZE: 742 amino acids; 81554 Da
      SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
      PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
      SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CD44 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2389947 2389947
      MOUSE ANTI-HUMAN CD44 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2459593 2459593
      MOUSE ANTI-HUMAN CD44 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2018060 2018060
      MOUSE ANTI-HUMAN CD44 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2234142 2234142
      MOUSE ANTI-HUMAN CD44 R-PE CONJUGATED MONOCLONAL ANTIBODY -2669925 2669925

      References

      Reference overviewPub Med ID
      Identification of mRNA that encodes an alternative form of H-CAM(CD44) in lymphoid and nonlymphoid tissues.
      Goldstein, L A and Butcher, E C
      Immunogenetics, 32: 389-97 (1990)  1990

      Show Abstract
      2272659 2272659

      Data Sheet

      Title
      Anti-H-CAM, Clone F10-44-2, Phycoerythrin Conjugated - Data Sheet

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      Alternative Format

      Catalogue Number Description  
      CBL154 Anti-H-CAM Antibody, clone F10-44-2 Show Pricing & Availability
      CBL154F Anti-H-CAM Antibody, clone F10-44-2, FITC conjugated Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies