05-904 | Anti-HA Tag Antibody

05-904
200 µg  
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      Overview

      Replacement Information
      Replacement Information05-904 is a recommended replacement for 07-221

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-904
      Replaces07-221
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HA Tag Antibody
      OverviewHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      Background InformationHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      References
      Product Information
      FormatPurified
      Control
      • RIPA lysates from HEK293 cells.
      PresentationPurified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
      Applications
      ApplicationDetect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.

      Immunocytochemistry:
      5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
      IsotypeIgG3
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Molecular WeightVarious
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HA Tag (mouse monoclonal IgG3) - DAM1462563 DAM1462563
      Anti-HA Tag - 2391105 2391105
      Anti-HA Tag - 2452520 2452520
      Anti-HA Tag - 0606033551 0606033551
      Anti-HA Tag - 0608038421 0608038421
      Anti-HA Tag - 0610043994 0610043994
      Anti-HA Tag - 0611045941 0611045941
      Anti-HA Tag - 1984837 1984837
      Anti-HA Tag - 2044818 2044818
      Anti-HA Tag - 2064516 2064516

      References

      Reference overviewApplicationSpeciesPub Med ID
      STAT2/IRF9 directs a prolonged ISGF3-like transcriptional response and antiviral activity in the absence of STAT1.
      Blaszczyk, K; Olejnik, A; Nowicka, H; Ozgyin, L; Chen, YL; Chmielewski, S; Kostyrko, K; Wesoly, J; Balint, BL; Lee, CK; Bluyssen, HA
      The Biochemical journal  466  511-24  2015

      Show Abstract
      25564224 25564224
      p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death.
      Long, JS; Schoonen, PM; Graczyk, D; O'Prey, J; Ryan, KM
      Oncogene  34  5152-62  2015

      Show Abstract
      25659586 25659586
      A role for the ITAM signaling module in specifying cytokine-receptor functions.
      Bezbradica, JS; Rosenstein, RK; DeMarco, RA; Brodsky, I; Medzhitov, R
      Nature immunology  15  333-42  2014

      Show Abstract
      24608040 24608040
      A novel Netrin-1-sensitive mechanism promotes local SNARE-mediated exocytosis during axon branching.
      Winkle, CC; McClain, LM; Valtschanoff, JG; Park, CS; Maglione, C; Gupton, SL
      The Journal of cell biology  205  217-32  2014

      Show Abstract
      24778312 24778312
      AKT regulates NPM dependent ARF localization and p53mut stability in tumors.
      Hamilton, G; Abraham, AG; Morton, J; Sampson, O; Pefani, DE; Khoronenkova, S; Grawenda, A; Papaspyropoulos, A; Jamieson, N; McKay, C; Sansom, O; Dianov, GL; O'Neill, E
      Oncotarget  5  6142-67  2014

      Show Abstract
      25071014 25071014
      A rapid and efficient immunoenzymatic assay to detect receptor protein interactions: G protein-coupled receptors.
      Zappelli, E; Daniele, S; Abbracchio, MP; Martini, C; Trincavelli, ML
      International journal of molecular sciences  15  6252-64  2014

      Show Abstract
      24733071 24733071
      Resveratrol modulates mitochondria dynamics in replicative senescent yeast cells.
      Wang, IH; Chen, HY; Wang, YH; Chang, KW; Chen, YC; Chang, CR
      PloS one  9  e104345  2014

      Show Abstract
      Western Blotting25098588 25098588
      Histone variant Htz1 promotes histone H3 acetylation to enhance nucleotide excision repair in Htz1 nucleosomes.
      Yu, Y; Deng, Y; Reed, SH; Millar, CB; Waters, R
      Nucleic acids research  41  9006-19  2013

      Show Abstract
      23925126 23925126
      A RASSF1A polymorphism restricts p53/p73 activation and associates with poor survival and accelerated age of onset of soft tissue sarcoma.
      Yee, KS; Grochola, L; Hamilton, G; Grawenda, A; Bond, EE; Taubert, H; Wurl, P; Bond, GL; O'Neill, E
      Cancer research  72  2206-17  2012

      Show Abstract
      22389451 22389451
      Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.
      Kobayashi, H; Nolan, A; Naveed, B; Hoshino, Y; Segal, LN; Fujita, Y; Rom, WN; Weiden, MD
      Journal of immunology (Baltimore, Md. : 1950)  186  403-10  2011

      Show Abstract
      21098228 21098228