05-100 | Anti-HDAC1 Antibody, clone 2E10

05-100
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-100
      Replaces05-614
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HDAC1 Antibody, clone 2E10
      References
      Product Information
      FormatCulture Supernatant
      Presentation100μl of culture supernatant containing 0.05% sodium azide. Frozen at -20°C.
      Applications
      ApplicationDetect HDAC1 with Anti-HDAC1 Antibody, clone 2E10 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
      Clone2E10
      HostMouse
      SpecificityHDAC-1
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
      Gene Symbol
      • HDAC1
      • RPD3L1
      • GON-10
      • DKFZp686H12203
      • HD1
      • RPD3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13547 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      SIZE: 482 amino acids; 55103 Da
      SUBUNIT: Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, PCAF, PHB2, MIER1, JMJD2A/JHDM3A, MINT, NRIP1, RERE, SETDB1, SUV39H1, TGIF, TGIF2, UHRF1 and UHRF2. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and JARID1B. Interacts with SAP30L.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
      PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. & Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      SIMILARITY: SwissProt: Q13547 ## Belongs to the histone deacetylase family. Type 1 subfamily.
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 2116616 2116616
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 2455716 2455716
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 1962113 1962113
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 2023242 2023242
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 2196123 2196123
      Anti-HDAC1, clone 2E10 (mouse monoclonal IgG1, culture supernatant) - 2329124 2329124
      Anti-HDAC1, clone 2E10 - 2424699 2424699
      Anti-HDAC1, clone 2E10 - 2044187 2044187
      Anti-HDAC1, clone 2E10 - DAM1493435 DAM1493435
      Anti-HDAC1, clone 2E10 - DAM1621691 DAM1621691

      References

      Reference overviewApplicationPub Med ID
      Deacetylase inhibitors repress STAT5-mediated transcription by interfering with bromodomain and extra-terminal (BET) protein function.
      Pinz, S; Unser, S; Buob, D; Fischer, P; Jobst, B; Rascle, A
      Nucleic acids research  43  3524-45  2015

      Show Abstract
      25769527 25769527
      NFκB1 is a suppressor of neutrophil-driven hepatocellular carcinoma.
      Wilson, CL; Jurk, D; Fullard, N; Banks, P; Page, A; Luli, S; Elsharkawy, AM; Gieling, RG; Chakraborty, JB; Fox, C; Richardson, C; Callaghan, K; Blair, GE; Fox, N; Lagnado, A; Passos, JF; Moore, AJ; Smith, GR; Tiniakos, DG; Mann, J; Oakley, F; Mann, DA
      Nature communications  6  6818  2015

      Show Abstract
      25879839 25879839
      Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer.
      Shen, Q; Tang, W; Sun, J; Feng, L; Jin, H; Wang, X
      American journal of translational research  6  538-47  2014

      Show Abstract
      25360218 25360218
      Lysine deacetylase inhibition promotes relaxation of arterial tone and C-terminal acetylation of HSPB6 (Hsp20) in vascular smooth muscle cells.
      Chen, A; Karolczak-Bayatti, M; Sweeney, M; Treumann, A; Morrissey, K; Ulrich, SM; Europe-Finner, GN; Taggart, MJ
      Physiological reports  1  e00127  2013

      Show Abstract
      24400135 24400135
      Class I lysine deacetylases facilitate glucocorticoid-induced transcription.
      Kadiyala, V; Patrick, NM; Mathieu, W; Jaime-Frias, R; Pookhao, N; An, L; Smith, CL
      The Journal of biological chemistry  288  28900-12  2013

      Show Abstract
      23946490 23946490
      Efficient genetic manipulation of 1321N1 astrocytoma cells using lentiviral gene transfer.
      Anja Keim,Isabelle Müller,Gerald Thiel
      Journal of neuroscience methods  206  2012

      Show Abstract
      22387260 22387260
      Breast cancer cells induce stromal fibroblasts to secrete ADAMTS1 for cancer invasion through an epigenetic change.
      Tyan, SW; Hsu, CH; Peng, KL; Chen, CC; Kuo, WH; Lee, EY; Shew, JY; Chang, KJ; Juan, LJ; Lee, WH
      PloS one  7  e35128  2012

      Show Abstract
      22514714 22514714
      A transcriptional repressor co-regulatory network governing androgen response in prostate cancers.
      Chng, KR; Chang, CW; Tan, SK; Yang, C; Hong, SZ; Sng, NY; Cheung, E
      The EMBO journal  31  2810-23  2012

      Show Abstract
      Western Blotting22531786 22531786
      Neural differentiation of P19 carcinoma cells and primary neurospheres: cell morphology, proliferation, viability, and functionality.
      Priscilla D Negraes,Telma T Schwindt,Cleber A Trujillo,Henning Ulrich
      Current protocols in stem cell biology  Chapter 2  2012

      Show Abstract
      22415841 22415841
      A radiosensitizing effect of artesunate in glioblastoma cells is associated with a diminished expression of the inhibitor of apoptosis protein survivin.
      Sebastian Reichert,Vera Reinboldt,Stephanie Hehlgans,Thomas Efferth,Claus Rödel,Franz Rödel
      Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology  103  2012

      Show Abstract
      22560712 22560712

      FAQ

      QuestionAnswer
      How do I prepare a non-reducing sample buffer?Non-reducing sample buffer can be made and stored for months at room temperature. Here is the formulation for a 2X Non-reducing sample buffer: Water (37ml), 0.5M Tris-HCl (10ml), Glycerol (8ml), 10% SDS (16ml), 0.05% Bromophenol Blue (5ml).

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies