04-229 | Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal

04-229
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IHC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-229
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HDAC2 Antibody, clone Y461, rabbit monoclonal
      Alternate Names
      • YY1-associated factor 1
      • histone deacetylase 2
      • transcriptional regulator homolog RPD3
      Background InformationHistone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • K562 whole cell lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesFlow Cytometry:
      1:60 dilution of a previous lot was used on flow cytometry.

      Immunoprecipitation:
      1:60 dilution of a previous lot was used on immunoprecipitation.

      Immunocytochemistry:
      1:250-500 dilution of a previous lot was used in immunocytochemistry.

      Immunohistochemistry:
      1:250-500 dilution of a previous lot was used in immunohistochemistry.



      Biological Information
      ImmunogenKLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 2.
      EpitopeC-terminus
      CloneY461
      HostRabbit
      SpecificityThis antibody recognizes Histone Deacetylase 2 at the C-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human, mouse and rat HDAC2.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A,H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq].
      Gene Symbol
      • EC 3.5.1.98
      • HD2
      • OTTHUMP00000040427
      • RPD3
      • YAF1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92769 # Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1.

      SIZE: 488 amino acids; 55364 Da

      SUBUNIT: Interacts with the non-histone region of H2AFY (By similarity). Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, DNMT1, MINT, HDAC7, HDAC10, HCFC1, NRIP1, MJD2A/JHDM3A, SAP30, SETDB1 and SUV39H1. Interacts with the non-histone region of H2AFY. Interacts with PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with CBFA2T3. Interacts with SAP30L.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Widely expressed; lower levels in brain and lung.

      SIMILARITY: SwissProt: Q92769 ## Belongs to the histone deacetylase family. Type 1 subfamily.
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on K562 whole cell lysate.

      Western Blot Analysis:
      A 1:2000-1:10,000 dilution of this lot was used to detect Histone Deacetylase 2 on K562 whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC2, clone Y461 - 2378176 2378176
      Anti-HDAC2, clone Y461 - 2446688 2446688
      Anti-HDAC2, clone Y461 - 2453127 2453127
      Anti-HDAC2, clone Y461 - 2068519 2068519
      Anti-HDAC2, clone Y461 - 2161088 2161088
      Anti-HDAC2, clone Y461 - 2275531 2275531
      Anti-HDAC2, clone Y461 - DAM1621688 DAM1621688
      Anti-HDAC2, clone Y461 - NG1572368 NG1572368
      Anti-HDAC2, clone Y461 - NG1853926 NG1853926

      References

      Reference overviewPub Med ID
      HDAC2 blockade by nitric oxide and histone deacetylase inhibitors reveals a common target in Duchenne muscular dystrophy treatment.
      Colussi, Claudia, et al.
      Proc. Natl. Acad. Sci. U.S.A., 105: 19183-7 (2008)  2008

      Show Abstract
      19047631 19047631
      HDAC2 deficiency and histone acetylation.
      Ree, Anne Hansen, et al.
      Nat. Genet., 40: 812-3; author reply 813 (2008)  2008

      18583969 18583969
      S-Nitrosylation of histone deacetylase 2 induces chromatin remodelling in neurons.
      Nott, Alexi, et al.
      Nature, 455: 411-5 (2008)  2008

      18754010 18754010