07-937 | Anti-HDAC7 Antibody

07-937
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-937
      DescriptionAnti-HDAC7 Antibody
      Alternate Names
      • histone deacetylase 7
      • histone deacetylase 7A
      Background InformationHistone deacetylases (HDAC) are enzymes that regulates transcription by selectively deacetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of the HDAC family have been identified, divided in to two classes. Class I includes HDAC-1, 2, 3 and 8 while Class II includes HDAC-4, 5, 6, and 7. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a C-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional co-repressor complexes that can be recruited to specific promoter regions via interactions with transcription factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones, resulting in a tighter DNA-histone interaction. This compaction of chromatin leads to the repression of transcription of DNA in that region.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Recombinant HDAC7 protein (Cat. #14-832).
      PresentationPurified Rabbit polyclonal in buffer containing 20 mM PBS and 0.1% sodium azide.
      Applications
      ApplicationUse Anti-HDAC7 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect HDAC7 also known as histone deacetylase 7, histone deacetylase 7A.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting:
      A previous lot of this antibody was shown to recognize recombinant HDAC7 but not recombinant HDAC1, HDAC4, or HDAC8.

      Western Blotting:
      A previous lot of this antibody was shown to recognize endogenous HDAC7 in HEK293T cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 164-182 of human HDAC7 [(C)KPKKSLERRKNPLLRKESA].
      Epitopea.a.164-182
      HostRabbit
      SpecificityThis antibody recognizes human HDAC7. Does not cross-react with HDAC1, HDAC4, or HDAC8.
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with Human. Not tested with other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq].
      Gene Symbol
      • DKFZP586J0917
      • EC 3.5.1.98
      • FLJ99588
      • HD7
      • HD7A
      • HD7a
      • HDAC7A
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors By similarity. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.

      Catalytic activity Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

      SUBUNIT: Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C By similarity. Interacts with HTATIP and EDNRA. Interacts with JARID1B.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note= In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.

      DOMAIN:The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm By similarity.

      PTM: May be phosphorylated by CaMK1 By similarity.

      MISCELLANEOUS: Its activity is inhibited by Trichostatin A (TSA), a known histone deacetylase inhibitor By similarity.

      SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.

      Molecular Weight100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western blotting using recombinant HDAC7 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC7 - 2469368 2469368
      Anti-HDAC7 - DAM1706875 DAM1706875
      Anti-HDAC7 - NG1564049 NG1564049
      Anti-HDAC7 -2493969 2493969
      Anti-HDAC7 -2526504 2526504
      Anti-HDAC7 -2844446 2844446
      Anti-HDAC7 Polyclonal Antibody 2884641

      References

      Reference overviewPub Med ID
      Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
      Gao, Chengzhuo, et al.
      Mol. Cell. Biol., 28: 5658-67 (2008)  2008

      Show Abstract
      18625722 18625722
      Histone deacetylase inhibitors selectively suppress expression of HDAC7.
      Dokmanovic, Milos, et al.
      Mol. Cancer Ther., 6: 2525-34 (2007)  2007

      Show Abstract
      17876049 17876049
      HDAC7, a thymus-specific class II histone deacetylase, regulates Nur77 transcription and TCR-mediated apoptosis.
      Dequiedt, Franck, et al.
      Immunity, 18: 687-98 (2003)  2003

      Show Abstract
      12753745 12753745