05-897 | Anti-HDAC9 Antibody, clone LH/JC2

05-897
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-897
      ReplacesMABE562
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HDAC9 Antibody, clone LH/JC2
      References
      Product Information
      FormatPurified
      Applications
      ApplicationDetect HDAC9 also known as histone deacetylase 9 with Anti-HDAC9 Antibody, clone LH/JC2 (Rat Monoclonal Antibody), that has been demonstrated to work in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      Immunogensynthetic peptide corresponding to amino acids 1046-1060 of human HDAC9 isoform 5
      Cloneclone LH/JC2
      HostRat
      SpecificityHDAC9
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
      Gene Symbol
      • HDAC9
      • HDAC9FL
      • MITR
      • HD9
      • HDRP
      • HDAC9B
      • KIAA0744
      • DKFZp779K1053
      • HDAC
      • HD7B
      • HD7
      • HDAC7B
      • HDAC7
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UKV0 # Isoform 3, called MITR/HDRP, lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting c-Jun phosphorylation by MAPK10 and by repressing c-Jun transcription via HDAC1 recruitment to c-Jun promoter.
      SIZE: 1011 amino acids; 111297 Da
      SUBUNIT: Homodimer. Interacts with CTBP1. The phosphorylated form interacts with 14-3-3 (By similarity). Interacts with HDAC1 and HDAC3, and probably with HDAC4 and HDAC5. Interacts with MEF2, MAPK10, ETV6, NCOR1 and BCL6.
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      TISSUE SPECIFICITY: Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
      PTM: Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3- binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. & Sumoylated.
      DISEASE: SwissProt: Q9UKV0 # A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly [MIM:604229]. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
      SIMILARITY: SwissProt: Q9UKV0 ## Belongs to the histone deacetylase family. Type 2 subfamily.
      Molecular Weight160kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot with RIPA lysates from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC9, clone LH/JC2 (rat monoclonal IgM) - 2198738 2198738
      Anti-HDAC9, clone LH/JC2 - 30364 30364

      References

      Reference overviewPub Med ID
      The histone deacetylase 9 gene encodes multiple protein isoforms.
      Petrie, Kevin, et al.
      J. Biol. Chem., 278: 16059-72 (2003)  2003

      Show Abstract
      12590135 12590135

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies