05-588 | Anti-HET/SAF-B Antibody, clone 6F7

05-588
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-588
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HET/SAF-B Antibody, clone 6F7
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-HET/SAF-B Antibody, clone 6F7 is a high quality Mouse Monoclonal Antibody for the detection of HET/SAF-B & has been validated in IP, WB, ICC & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Biological Information
      ImmunogenPeptide corresponding to residues 345-357 of human HET/SAF-B
      Cloneclone 6F7
      HostMouse
      SpecificityHET/SAF-B
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.
      Gene Symbol
      • SAFB
      • HET
      • DKFZp779C1727
      • HAP
      • SAF-B
      • SAFB1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15424 # Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
      SIZE: 915 amino acids; 102642 Da
      SUBUNIT: Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and SFRS10 (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
      SIMILARITY: SwissProt: Q15424 ## Contains 1 RRM (RNA recognition motif) domain. & Contains 1 SAP domain.
      Molecular Weight130kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on whole cell extracts or RIPA lysates from MCF-7 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HET/SAF-B, clone 6F7 2472930
      Anti-HET/SAF-B, clone 6F7 (mouse monoclonal IgG) 2905305
      Anti-HET/SAF-B, clone 6F7 - 20235 20235
      Anti-HET/SAF-B, clone 6F7 - 27806 27806

      References

      Reference overviewApplicationPub Med ID
      The chromatin scaffold protein SAFB1 localizes SUMO-1 to the promoters of ribosomal protein genes to facilitate transcription initiation and splicing.
      Liu, HW; Banerjee, T; Guan, X; Freitas, MA; Parvin, JD
      Nucleic acids research  43  3605-13  2015

      Show Abstract
      25800734 25800734
      B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress.
      Werwein, E; Dzuganova, M; Usadel, C; Klempnauer, KH
      Cell death & disease  4  e511  2013

      Show Abstract
      Immunofluorescence23449447 23449447
      HOXC6 Is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor in an estrogen environment.
      Ansari, KI; Hussain, I; Shrestha, B; Kasiri, S; Mandal, SS
      Journal of molecular biology  411  334-49  2011

      Show Abstract
      21683083 21683083
      SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor.
      Townson, Steven M, et al.
      J. Biol. Chem., 278: 20059-68 (2003)  2003

      Show Abstract
      Immunoblotting (Western)12660241 12660241
      Tamoxifen-bound estrogen receptor (ER) strongly interacts with the nuclear matrix protein HET/SAF-B, a novel inhibitor of ER-mediated transactivation.
      Oesterreich, S, et al.
      Mol. Endocrinol., 14: 369-81 (2000)  2000

      Show Abstract
      10707955 10707955