06-478 | Anti-HSP27 Antibody

06-478
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-478
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HSP27 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide
      Applications
      ApplicationAnti-HSP27 Antibody detects level of HSP27 & has been published & validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenRecombinant human HSP27
      HostRabbit
      SpecificityHSP27
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPB1
      • Hs.76067
      • HSP27
      • Hsp25
      • HspB1
      • HSP28
      • CMT2F
      • HS.76067
      • DKFZp586P1322
      • HMN2B
      • SRP27
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
      SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
      PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
      DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
      SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
      Molecular Weight27kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on cell lysates of heat shocked human MCF7 cells or in cell lysates of heat shocked human HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HSP27 (rabbit polyclonal IgG) - 2138413 2138413
      Anti-HSP27 (rabbit polyclonal IgG) - 2147325 2147325
      Anti-HSP27 (rabbit polyclonal IgG) - 2410235 2410235
      Anti-HSP27 (rabbit polyclonal IgG) - 2189945 2189945
      Anti-HSP27 (rabbit polyclonal IgG) - 2283381 2283381
      Anti-HSP27 - 14533 14533
      Anti-HSP27 - 22714 22714
      Anti-HSP27 - 25912 25912
      Anti-HSP27 - 29457 29457
      Anti-HSP27 - DAM1734761 DAM1734761

      References

      Reference overviewApplicationSpeciesPub Med ID
      Rottlerin-mediated inhibition of Chlamydia trachomatis growth and uptake of sphingolipids is independent of p38-regulated/activated protein kinase (PRAK).
      Lei, L; Li, Z; Zhong, G
      PloS one  7  e44733  2012

      Show Abstract
      Western Blotting22970301 22970301
      Acetylation of heat shock protein 20 (Hsp20) regulates human myometrial activity.
      Karolczak-Bayatti, M; Sweeney, M; Cheng, J; Edey, L; Robson, SC; Ulrich, SM; Treumann, A; Taggart, MJ; Europe-Finner, GN
      The Journal of biological chemistry  286  34346-55  2011

      Show Abstract
      Immunoprecipitation21803775 21803775
      Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with components of the actin and intermediate filament cytoskeleton.
      Yifeng Jia,Shiaw-Lin Wu,Jeff S Isenberg,Shujia Dai,John M Sipes,Lyndsay Field,Bixi Zeng,Russell W Bandle,Lisa A Ridnour,David A Wink,Ramani Ramchandran,Barry L Karger,David D Roberts
      Cell stress & chaperones  15  2010

      Show Abstract Full Text Article
      19579057 19579057
      PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention.
      Yao, S; Bee, A; Brewer, D; Dodson, A; Beesley, C; Ke, Y; Ambroisine, L; Fisher, G; Møller, H; Dickinson, T; Gerard, P; Lian, LY; Risk, J; Lane, B; Smith, P; Reuter, V; Berney, D; Gosden, C; Scardino, P; Cuzick, J; Djamgoz, MB; Cooper, C; Foster, CS
      Genes & cancer  1  444-64  2010

      Show Abstract
      Western BlottingHuman21779455 21779455
      Evidence that a protein kinase A substrate, small heat-shock protein 20, modulates myometrial relaxation in human pregnancy.
      Tyson, EK; Macintyre, DA; Smith, R; Chan, EC; Read, M
      Endocrinology  149  6157-65  2008

      Show Abstract
      Western Blotting18755793 18755793
      The small heat shock-related protein, HSP20, is phosphorylated on serine 16 during cyclic nucleotide-dependent relaxation
      Beall, A., et al
      J Biol Chem, 274:11344-51 (1999)  1999

      Immunoblotting (Western)10196226 10196226
      Prognostic significance of heat-shock protein-27 in node-positive breast carcinoma: an immunohistochemical study.
      Têtu, B, et al.
      Breast Cancer Res. Treat., 36: 93-7 (1995)  1995

      Show Abstract
      7579511 7579511
      Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27.
      Lavoie, J N, et al.
      Mol. Cell. Biol., 15: 505-16 (1995)  1995

      Show Abstract
      7799959 7799959
      Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells.
      Landry, J, et al.
      J. Cell Biol., 109: 7-15 (1989)  1989

      Show Abstract
      2745558 2745558
      Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein.
      Arrigo, A P, et al.
      Mol. Cell. Biol., 8: 5059-71 (1988)  1988

      3072471 3072471

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies